Invitrogen™ TGF alpha Human ELISA Kit

The Human Transforming Growth Factor (TGFα) ELISA quantitates Hu TGFα in human serum, plasma, or cell culture medium.

Supplier:  Invitrogen™ EHTGFAX5

Catalog No. EHTGFAX5

Description

The Human Transforming Growth Factor (TGFα) ELISA quantitates Hu TGFα in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TGFα. Principle of the method The Human TGFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Specifications

• Accession Number: EGF-like TGF; ETGF; LOC100009482; protransforming growth factor alpha; RATTGFAA; TFGA; TGF a; TGF type 1; TGF a; Tgfa; TGFAA; TGFalpha; TGF-alpha; TGFa; Transforming growth factor; Transforming growth factor alpha; transforming growth factor, alpha; transforming growth factor-alpha; wa1; wa-1; waved 1
• Assay Sensitivity: 3 pg/mL
• Product Type: ELISA
• Gene ID (Entrez): P01135
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate
  Standard
  Assay Diluent concentrate
  Biotinylated Detection Antibody
  SAV-HRP
  Wash Buffer
  Chromogen
  Stop Solution
  Adhesive Plate Covers
• Quantity: 5 x 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
• Target: TGF alpha
• Total Assay Time: 4 hr 45 min

• Assay Range: 1.37 to 1000 pg/mL
• Conjugate: Biotin
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: TGFA
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: Research Use Only
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min