Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Includes: Detection Reagent 1 and 2

Catalog Number	Mfr. No.	Quantity	Sufficient For
PI80196	Thermo Scientific™ 80196	100 mL	10 Mini-gel blots, 1000cm2 of membrane
PI80196X3	Thermo Scientific™ 80196X3	3 x 100 mL	30 Mini-gel blots, 3000cm2 of membrane
PI80197	Thermo Scientific™ 80197	25 mL	250cm2 of membrane

Description

Pierce ECL 2 Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL 2 Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers.

Pierce ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate.

Highlights:

• High sensitivity — detect targets down to the low-picogram level
• Long signal duration — sustained light output for as long as five hours
• More imaging options — X-ray, CCD or laser-based imagers
• More affordable — high quality and performance at a lower price than other suppliers' similar substrates

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Specifications

• Product Type: ECL Western Blot Substrate
• Kit Contents: Detection reagent 1 and 2
• Content And Storage: 2°C to 8°C