Thymidine, MP Biomedicals™

Used in forming hybrid cell lines where cells are incubated in HAT medium. Thymidine, MP Biomedicals, a cell culture reagent, is a nucleoside composed of deoxyribose (a pentose sugar) joined to the pyrimidine base thymine. It is highly stable and appears

Mainly used in forming hybrid cell lines using HAT (hypoxanthine-aminopterin-thymidine) medium to give rise to monoclonal antibodies. Also used in HT and HAT cocktails for hybridoma fusion, selection and cloning.

Deoxythymidine can be phosphorylated with one, two or three phosphoric acid groups, creating respectively dTMP, dTDP or dTTP (deoxythymidine mono-, di-, or triphosphate). The stability of deoxythymidine under standard temperature and pressure (STP) is very high. Thymidine is listed as a chemical teratogen.

Chemical Identifiers

CAS

50-89-5

Molecular Formula

C10H14N2O5

Molecular Weight (g/mol)

242.231

InChI Key

IQFYYKKMVGJFEH-XLPZGREQSA-N

Synonym

thymidine, deoxythymidine, 2'-deoxythymidine, 5-methyldeoxyuridine, thymidin, beta-thymidine, dthyd, 5-methyl-2'-deoxyuridine, thymine-2-deoxyriboside, thyminedeoxyriboside

PubChem CID

5789

ChEBI

CHEBI:17748

IUPAC Name

1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione

SMILES

CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)O

Melting Point

185°C to 191°C

Quantity

25g

Solubility Information

Soluble in water, methanol, hot alcohol, hot acetone, hot ethyl acetate pyridine, glacial acetic acid and HCl (20mg/mL, clear, colorless solution); sparingly soluble in hot chloroform.

Formula Weight

242.2

Optical Rotation

17.5–19.5° (c=1, water)

Physical Form

White Powder

Percent Purity

>99.9%

Recommended Storage

Store at Room Temperature (15–30°C)

Recommended Storage

Store at Room Temperature (15–30°C)