Thymidine, MP Biomedicals™

Description

Used in forming hybrid cell lines where cells are incubated in HAT medium. Thymidine, MP Biomedicals, a cell culture reagent, is a nucleoside composed of deoxyribose (a pentose sugar) joined to the pyrimidine base thymine. It is highly stable and appears

Mainly used in forming hybrid cell lines using HAT (hypoxanthine-aminopterin-thymidine) medium to give rise to monoclonal antibodies. Also used in HT and HAT cocktails for hybridoma fusion, selection and cloning.

Deoxythymidine can be phosphorylated with one, two or three phosphoric acid groups, creating respectively dTMP, dTDP or dTTP (deoxythymidine mono-, di-, or triphosphate). The stability of deoxythymidine under standard temperature and pressure (STP) is very high. Thymidine is listed as a chemical teratogen.

Specifications

Chemical Identifiers

• CAS: 50-89-5
• Molecular Formula: C10H14N2O5
• Molecular Weight (g/mol): 242.231
• InChI Key: IQFYYKKMVGJFEH-XLPZGREQSA-N
• Synonym: thymidine, deoxythymidine, 2'-deoxythymidine, 5-methyldeoxyuridine, thymidin, beta-thymidine, dthyd, 5-methyl-2'-deoxyuridine, thymine-2-deoxyriboside, thyminedeoxyriboside
• PubChem CID: 5789
• ChEBI: CHEBI:17748
• IUPAC Name: 1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
• SMILES: CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)O

• Melting Point: 185°C to 191°C
• Quantity: 1g
• Solubility Information: Soluble in water, methanol, hot alcohol, hot acetone, hot ethyl acetate pyridine, glacial acetic acid and HCl (20mg/mL, clear, colorless solution); sparingly soluble in hot chloroform.
• Formula Weight: 242.2
• Optical Rotation: 17.5–19.5° (c=1, water)
• Physical Form: White Powder
• Percent Purity: >99.9%

Safety and Handling

Recommended Storage

Store at Room Temperature (15–30°C)