Invitrogen™ Thyroxine (T4) Competitive ELISA Kit

Includes

Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.

Competitive ELISA

The Thyroxine (T4) ELISA quantitates Thyroxine (T4) in serum, plasma, urine, dried fecal extracts, or cell culture medium. The assay will exclusively recognize both natural and recombinant Thyroxine (T4). Principle of the method The Thyroxine (T4) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Thyroxine is the main hormone produced by the thyroid gland. The thyroid hormones, triiodothyronine (T3) and thyroxine (T4), are tyrosine-based hormones produced by the thyroid gland that are primarily responsible for regulation of metabolism. Iodine is necessary for the production of T3 and T4. The major form of thyroid hormone in the blood is thyroxine, which has a longer half-life than T3. T4 is converted to the active T3 within cells by deiodinases (5’-iodinase). These are further processed by decarboxylation and deiodination to produce iodothyronamine and thyronamine. Thyroid hormone production is regulated via pituitary thyrotropin (TSH) modulation of thyroxine prohormone secretion by the thyroid gland and regulation of active triiodothyronine production. Thyroxine is identical across all species.

Assay Range

0.625-20 ng/mL

Assay Sensitivity

0.29 ng/mL

Description

Thyroxine (T4) Competitive ELISA Kit

Assay

Multiplex assay on the Luminex Platform

Conjugate

HRP

Detection Method

Fluorescence

Product Type

ELISA Kit

Protein Family

Thyroid hormone

Sample Type

Fecal Extract, Plasma, Serum, Supernatant, Urine

Sufficient For

96 assays

Target Species

Human

For Use With (Application)

ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti

For Use With (Equipment)

Colorimetric Microplate Reader

Interassay CV

7%

Intraassay CV

3%

Kit Contents

Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers

Label or Dye

HRP

Quantity

96 Tests

Regulatory Status

RUO

Research Discipline

Metabolism

Sample Volume

Fecal Extract,10 μL; Plasma, 1 μL; Serum, 1 μL; Supernatant, 10 μL; Urine,2.5 μL

Storage Requirements

-20°C

Target

Chemical

Test Time

1 hr. 20 min.

Total Assay Time

1 hr. 30 min.