Thermo Scientific™ TMT™ Elution Buffer and Anti-TMT Resin

For enhanced enrichment, use Thermo Scientific TMT Elution Buffer with Immobilized Anti-TMT Resin for peptides that have been chemically labeled with Tandem Mass Tag (TMT) Isobaric Label Reagents for multiplexed protein quantitation using tandem mass spectrometry.

Tandem mass tag (TMT) isobaric label reagents are used for multiplexed protein quantitation using tandem mass spectrometry. Immobilized Anti-TMT Resin is designed to effectively capture and enrich peptides or proteins labeled using any TMT reagent. Anti-TMT Resin and TMT Elution Buffer are effective for reducing sample complexity, improving dynamic range, and studying cysteine modifications such as S-nitrosylation, oxidation, and disulfide bonds of protein samples labeled with iodoTMT reagents.

Tandem mass tag (TMT) reagents are used to chemically label proteins in different samples for multiplexed quantitation using tandem mass spectrometry. Immobilized Anti-TMT Resin contains an agarose-immobilized anti-TMT monoclonal antibody and is effective for capture and enrichment of peptides or proteins labeled using any TMT reagent. This helps simplify otherwise complex samples before LC-MS/MS analysis, especially when the targets for labeling are rare (e.g., cysteine sulfhydryls). Enrichment of labeled peptides by this method results in fewer peptides identified per protein, but can significantly increase the number of unique proteins identified, making it easier to detect and measure low-abundance proteins.

Immobilized Anti-TMT Resin uses our highly specific Anti-TMT Antibody (Cat. No. 90075) to bind peptides labeled with TMT reagents. The antibody is specific for the mass reporter region of the TMT reagents, which allows for enrichment of TMT-labeled peptides regardless of peptide labeling chemistry (e.g., amine-reactive, sulfhydryl-reactive). TMT Elution Buffer is a volatile neutral buffer that competitively elutes captured TMT-labeled peptides. When used in combination with Immobilized Anti-TMT Resin, TMT Elution Buffer provides higher specific enrichment of TMT-labeled peptides compared to acidic or basic elution buffers.

Enrichment features include:
• Specific—uses specific, high-affinity antibody for the TMT structure (Kd >100 pM)
• Compatible—effective for all varieties of TMT reagents (amine-reactive or cysteine-reactive)
• Versatile—use resin with TMT Elution Buffer for optimal sample enrichment

Description

Immobilized Anti-TMT™ Antibody Resin

Product Line

TMT™

Content And Storage

Upon receipt store all products at 4°C. Product shipped with an ice pack.

Label or Dye

Tandem Mass Tag™ (TMT) Labels

Starting Material

Cell Lysate

Detection Method

Mass Spectrometry

Workflow Step

Protein Labeling

Quantity

6 mL