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Invitrogen™ TrueCut™ HiFi Cas9 Protein (5 μg/μL)

A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
$277.14 - $970.08
Specifications
Concentration | 5 μg/μL |
---|---|
Purity | ≥90% |
Sensitive to Heat Inactivation | Yes |
Source | E. coli |
Product Type | HiFi Cas9 |
Description
- A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency
- Near complete elimination of off-target events in all tested cell lines including standard, immune, primary, and stem
- Maintains maximum on-target editing efficiency compared to TrueCut™ Cas9 Protein v2
- Transfection-ready using either electroporation or lipid-mediated transfection reagents
- Manufactured under strict ISO-13485 quality standards
- Ideal for applications that are sensitive to off-target events and when more precise editing is required
- Eliminates time consuming cloning steps
Available in Two Concentrations
- 1 μg/μL - For use in standard editing scenarios
- 5 μg/μL - For optimized editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
- For custom sizes or concentrations, please inquire
Specifications
5 μg/μL | |
Yes | |
HiFi Cas9 |
≥90% | |
E. coli | |
Liquid |
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