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ILEX LIFE SCIENCES LLC TRYPSIN/EDTA P10-028100 100ML
PAN-Biotech Trypsin 0.25% / 1 mM EDTA 4 Na in DPBS, w/o: Ca and Mg (100 ml). DESCRIPTION: Trypsin 0.25% / 1 mM EDTA 4 Na in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA-4Na in a PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: 0.042 % (1.00 mM) EDTA-Na4 x 2H2O. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
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