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- Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line
- They are a core component of the AAV-MAX Helper-Free AAV Production System
- The cells have been adapted to the chemically defined Viral Production Medium and are maintained in suspension culture in that medium
- Viral Production Cells 2.0 are highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production
- Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Viral Production Medium and 10% DMSO
- Cells should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance
Features of the Viral Production Cells 2.0 include:
- Clonal, 293F-derived, high-producer cell line
- Absence of SV40 large T antigen
- Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
- Enable production of high AAV titers, greater than 5 x 1010 vg/mL (unpurified)
- Robust scalability and passage stability
Specifications
Cell State
Frozen
Concentration
1 x 107 cells/mL
Content And Storage
1 cryovial, store in vapor phase liquid nitrogen
Cell Line
Viral Production Cells 2.0
Cell Type
Kidney (Embryonic)
Culture Type
Suspension Cell Culture
Form
Frozen
Species
Human
Format
1 vial
For Use With (Application)
AAV Production
For Research Use Only. Not for use in diagnostic procedures.
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