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![Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside],](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B71800-5.0.JPG-650.jpg)
Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 5 Grams
Chromogenic substrate of B-Galactosidase used in combination with IPTG for detection of B-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by B-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show B-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of B-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
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