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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 7 Peptide
SDP

Catalog No. PV3180
Encompass
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:
800 x 20μL assays

Description

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 7 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains a PKC consensus sequence (R⁄K)1-3(X)0-2 (S⁄T) (X)0-2(R⁄K)1-3, where X is any residue.

Specifications

Product Line Z´-LYTE™
Target Serine/Threonine Kinases
For Use With (Application) FRET
Assay Serine/Threonine Kinase Assay
Content And Storage Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Assay Entry Biochemical phospho-specific activity (no antibody)
Label or Dye Methylcoumarin
Product Type Kinase Assay Kit
Quantity 800 x 20 μL Assays
Format 384-well plate
Sample Type PKC consensus sequence (R⁄K)1-3(X)0-2 S⁄T (X)0-2(R⁄K)1-3
Excitation/Emission 405⁄465, 535
No. of Reactions 800 Reactions
Readout End Point
Shipping Condition Dry Ice
Substrate Peptide-Based Substrate
Target Entry MAP4K5, IRAK4, PRKCQ, NEK9, PRKCD, MAP3K9, PASK, PIM2, NEK2, PRKCG, SRPK2, STK4, NEK1, MAP4K2, MINK1, CHEK2, PIM1, PKN1, PRKCA, PRKCI, TAOK2, MAP4K4, RPS6KB1, PHKG1, PRKCZ, ROCK1, PHKG2, PRKCB1, STK24, STK3, PRKCB2, STK25, PRKCE, PRKCH, MST4
Validated Kinases ROCK1, RPS6KB1, SRPK2, STK3, STK4, STK24, STK25, TAOK2, NEK2, NEK9, PASK, PHKG1, PHKG2, PIM1, PIM2, PKN1, PKC, CHEK2, IRAK4, MAP3K9, MAP4K2, MAP4K4, MAP4K5, MINK1, MST4, NEK1
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For Research Use Only. Not for use in diagnostic procedures.

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