Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Tyrosine 6 Peptide

Z'-LYTE™ Kinase Assay Kit - Tyrosine 6 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for EGF Receptor.

Product Line

Z´-LYTE™

Target

Tyrosine Kinases

For Use With (Application)

FRET

Assay

Tyrosine Kinase Assay

Content And Storage

Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

Assay Entry

Biochemical phospho-specific activity (no antibody)

Label or Dye

Methylcoumarin

Product Type

Kinase Assay Kit

Quantity

800 x 20 μL Assays

Format

384-well plate

Sample Type

EGFR substrate (EEEEYFELVAKKK)

Excitation/Emission

405⁄465, 535

No. of Reactions

800 Reactions

References

Nature, 373, pp. 536-9 (1995), JBC 277, pp 1576-1585 (2002)

Readout

End Point

Shipping Condition

Dry Ice

Substrate

Peptide-Based Substrate

Target Entry

KIT, JAK1, JAK2 JH1 JH2, JAK2 JH1 JH2 V617F, ERBB2, MST1R, LTK, JAK2, AXL

Validated Kinases

AXL, ERBB2, JAK1, JAK2, KIT, LTK, MST1R

For Research Use Only. Not for use in diagnostic procedures.