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Invitrogen™ Zenon™ Mouse IgG1 Labeling Kits

Includes
Contains 1 vial of Zenon Alexa Fluor 594 mouse IgG1 labeling reagent (250 µL), and 1 vial of Zenon blocking reagent (mouse IgG, 250 µL).
Simplify your multicolor staining and flow cytometry experiments with our Zenon™ mouse IgG1 labeling kits. These antibody labeling kits are ideal for flow cytometry (e.g., FACS) experiments because they enable the simultaneous labeling of different target cells and tissues with differently labeled mouse monoclonal antibodies in a single staining protocol. Multiple Zenon™ antibody complexes may be prepared individually and used in a multicolor stain after using the Zenon™ blocking reagent.
Zenon labeling technology greatly simplifies the use of multiple mouse-derived antibodies in the same staining protocol. Zenon tricolor labeling kits contain sufficient materials for 10 labeling reactions of each of three different fluorescent colors.
Important features of Zenon labeling technology:
• Labeled antibodies are typically ready to use in 10 minutes
• Requires only 1–20 µg primary antibody
• Simple—no purification required
• Flexible — choose from different fluorophores, biotin, HRP, alkaline phosphatase
• Multiplex with other mouse monoclonal antibodies simultaneously
• Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging.
Advantages of using Zenon antibody labeling kits:
Cost savings
Zenon antibody labeling kits offer a cost-conscious and reproducible method of tagging as little as 0.4 µg in 2 µL of primary antibody, with minimal waste of expensive or difficult-to-obtain antibodies, or excessive washing steps that pose the risk of product loss.
Sensitivity
Label your primary antibodies without compromising their antigen binding affinity: Zenon dye- and enzyme-labeled Fab fragments, which are targeted to the Fc tail, are affinity purified during their preparation to ensure high affinity and selectivity for the Fc portion of the corresponding primary antibody. The procedure for chemical labeling of the Zenon Fab fragments protects their Fc-binding site, resulting in more active labeling reagents.
Speed
No purification procedure is required prior to using Zenon Fab fragments in your laboratory applications. Formation of the Fab-antibody complex occurs in fewer than five minutes, followed by a five-minute blocking step. During this time, almost all the primary antibody in the mixture is labeled with the labeled Fab fragments.
Simplicity
The Fab-antibody complexes display fluorescence or enzymatic activity that is similar in intensity to that of directly labeled primary antibodies. Varying the extent of the antibody labeling is as simple as changing the amount of added Zenon labeling reagent during the reaction. Once the labeling complexes are formed, they can used immediately, without need for antibody purification.
Reliability
The Zenon Fab-antibody complex is stable and allows subsequent or simultaneous labeling of different target cells and tissues with different complexes. After staining, an aldehyde-based fixing step may be used to prevent the transfer of different labels between different primary antibodies, preserving the initial staining pattern.
Customization
We offer custom antibody conjugation services that are efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.
Specifications
Quantity
50 Reactions
Content And Storage
Contains 1 vial of Zenon Alexa Fluor 594 mouse IgG1 labeling reagent (250 µL), and 1 vial of Zenon blocking reagent (mouse IgG, 250 µL).
Store in refrigerator (2–8°C) and protect from light.
Excitation/Emission
590/617
Labeling Scale
< 1–20 μg
Product Line
Zenon
Label or Dye
Alexa Fluor 594
Label Type
Alexa Fluor
Color
Red
Labeling Target
IgG1
Species
Mouse
For Research Use Only. Not for use in diagnostic procedures.
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