Invitrogen™ Zenon™ pHrodo™ iFL IgG Labeling Reagents

Zenon™ labeling technology provides a fast, versatile, and reliable method for adding a fluorescent label to an antibody. Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum.

Achieve rapid, scalable, and efficient labeling of internalized antibody with the Invitrogen™ Zenon™ pHrodo™ iFL IgG Labeling Reagents. These pH-sensitive dye conjugates are non-fluorescent outside the cell but fluoresce brightly red or green when inside acidic environments, including those of phagosomes. As a result, pHrodo iFL IgG Labeling Reagents can offer faster and more accurate results than any other phagocytosis assay.

Zenon™ pHrodo™ iFL Green-labeled or Red-labeled Fab fragments (i.e. labeling reagent) are directed against the Fc portion of an intact IgG primary antibody to form a labeling complex. Formation of the Fab–antibody complex occurs in fewer than 5 minutes. Because the pHrodo™ iFL dyes dramatically increase in fluorescence as the pH of their surroundings becomes more acidic, the antibodies coupled with the Zenon™ pHrodo™ iFL IgG Labeling Reagents provide an excellent measure of endocytic activity based on the acidification of the labeled antibodies as they are ingested by the cell.

The Zenon™ pHrodo™ iFL IgG Labeling Reagents contain sufficient material to label one to four 96-well plates, depending on the Zenon™ labeling reagent concentration used. Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.

Other advantages of the pH-sensitive Zenon™ pHrodo™ iFL IgG Labeling Reagents include:

• Simplicity — No reagent purification is required
• Accuracy — No wash steps or quenchers are needed, minimizing cross-reactivity or background
• Speed — Labeled antibodies are typically ready to use in 10 minutes
• Specificity — Dyes are designed for the specific detection of phagocytosis and endocytosis


Preserve primary antibody function and affinities
Reactive dye labeling of primary antibodies can have unpredictable and undesirable outcomes. One of these is reduced binding affinity due to label addition in the binding pocket. The Zenon™ antibody labeling approach, which is targeted to the Fc tail, resolves this potential issue.

Try custom antibody conjugates
If the antibody conjugate you need is not available as one of our stocked products, we will prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Order Info

Store in refrigerator at 2–8°C.

Description

pHrodo™ iFL Red, 250 μL

Kit Contents

1 vial of Zenon™ pHrodo™ iFL Red Human labeling reagent (250 μL)

Product Type

Labeling Reagent

Quantity

250 μL

Content And Storage

Store in refrigerator (2°C to 8°C) and protect from light

Excitation/Emission

560/585 nm

Product Line

Zenon™

Label or Dye

pHrodo iFL Red

Color

Red

Labeling Target

IgG

Species

Human

Label Type

pHrodo™ (pH sensitive)