ZeptoMetrix OXItek Glutathione Reductase Assay Kit

Includes

120mL of GR-assay buffer, 4 vials of GR-reagent 1, 4 vials of GR-reagent 2, 25mL of GR-reagent 3, 1 vial of QC material.

Provides an indirect and highly reproducible method of quantifying the activity of total cellular glutathione reductase

Glutathione reductase (GR, EC No. 1.6.4.2) is a flavoprotein that is required for the conversion of oxidized glutathione (GSSG) to reduced glutathione (GSH). At the same time, it oxidizes nicotinamide adenine dinucleotide phosphate (NADPH). This universally
present enzyme is essential for the maintenance of reduced glutathione (GSH) levels in vivo. Reduced glutathione plays an important role in oxidoreduction processes and detoxification of H₂O₂ and organic peroxides, which are substances produced in large quantities during inflammatory processes in living cells. Glutathione reductase therefore plays a major role in glutathione peroxidase (GPx) and glutathione stransferase (GST) reactions as an adjunct in the control of peroxides and free radicals.When levels of catalase, another universally present antioxidant enzyme, are decreased, the glutathione dependant enzymes become activated. A deficiency of glutathione reductase is characterized by hemolysis owing to the increased sensitivity of erythrocyte (RBC) membranes to H₂O₂ that lead to osmotic fragility. This reaction is thus required for the stability and integrity of red cells.

Certifications/Compliance

ISO 13485

Product Type

OXItek Glutathione Reductase assay Kit

Sufficient For

100 Tests

For Use With (Application)

For quantifying the activity of to tal cellular Glutathione Reductase

Kit Contents

GPx reagent 2 4 vial/kit, GPx-assay buffer 120 mL/kit, GPx-reagent 1 4 vial/kit, Gpx-reagent 3 25 mL/kit, QC material 1 vial/kit

Quantity

100 Tests

Storage Requirements

2°C to 4°C

Research purposes only. Not for in vitro diagnostic use.