ZeptoMetrix OXItek Total Glutathione Peroxidase Assay Kit

Description

Includes

120mL of GPx-assay buffer, 45mg of GPx-reagent 1, 5mg of GPx-reagent 2, 10mL of Gpx-reagent 3, 1mL of Gpx-Reagent 4, 0.5mL of QC material.

Provides a method of quantifying the activity of total glutathione peroxidase

Glutathione peroxidase (GPx, EC No. 1.11.1.9) is an enzyme found in cytoplasmic and mitochondrial fractions of cells. GPx acts on lipid hydroperoxide (LHP) substrates that are released from membrane phospholipids by phospholipase A2. It can utilize cholesterol hydroperoxide and hydrolyzes H₂O₂ at low concentrations. The antioxidant enzyme catalyzes the reduction of hydrogen peroxide and hydroperoxides formed from fatty acids, thereby effectively removing toxic peroxides from living cells. It
plays the important role of protecting cells from potential damage by free radicals, formed by peroxide decomposition. The activity of GPx is coupled to glutathione reductase (GSSG-R), which maintains reduced glutathione (GSH) levels. Using glutathione (GSH) as a reducing reagent, the GPx enzymes catalyze the reduction of H₂O₂ and organic peroxides (R-O-O-H) to water and the corresponding stable alcohol thus inhibiting the formation of free radicals. Enzyme activity can be decreased by
negative feedback from excess substrate or from damage by oxidative modification.

Specifications

• Certifications/Compliance: ISO 13485
• Product Type: OXItek Total Glutathione Peroxidase assay Kit
• Sufficient For: 100 Tests
• For Use With (Application): For quantifying the activity of to tal cellular Glutathione Reductase in a large number of biological specimen
• Kit Contents: 0.5mL QC material, 1 mL GPx-Reagent 4, 10 mL Gpx-Reagent 3, 120 mL GPx-Assay buffer, 45 mg GPx-Reagent 1, 5mg GPx reagent 2
• Quantity: 100 Tests
• Storage Requirements: 24°C to 25°C

Research purposes only. Not for in vitro diagnostic use.