Revvity Health Sciences Inc AlphaScreen Biotinylated-HIS

AlphaScreen and AlphaLISA are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym Alpha stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen i

Revvity Health Sciences Inc AlphaScreen P-Tyr-100 (Phosphotyrosine) Assay Kit, 500 assay points

AlphaScreen and AlphaLISA are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym Alpha stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen i

Revvity Health Sciences Inc AlphaScreen cAMP Detection Kit, 1,000 assay points

AlphaScreen no-wash assay kit for quantitative detection of cAMP. In this AlphaScreen competition assay, a biotinylated cAMP tracer binds to Streptavidin-coated Donor beads, and interacts with anti-cAMP antibody conjugated to AlphaScreen Acceptor beads. When no cAMP is present, the beads come into close proximity. The excitation of the Donor beads triggers the release of singlet oxygen molecules, which diffuse to the Acceptor beads, resulting in light emission at 520-620 nm. With increasing concentrations of endogenous cAMP, fewer Donor and Acceptor beads are brought together, resulting in a decrease in signal. This kit contains enough reagents to run 1,000 wells using a 25 'L reaction volume. Protocol can be found on the Certificate of Analysis. This product replaces 6760625D.

Revvity Health Sciences Inc AlphaScreen C-MYC Detection Kit, 500 assay points

This bead kit can be used for many applications, including: Protein-protein interaction assays Protein-peptide interaction assays C-myc tagged protein detection and quantitation Protein expression Binding studies In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is c-myc-tagged, and associates with the AlphaScreen anti-c-myc Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated c-myc probe provided in the kit.