A plasmid vector for Acinetobacter calcoaceticus or Escherichia coli. The cryptic plasmid pWH1277 isolated from A. colcoaceticus lwoffi was linearized with PvuII and cloned into the PvuII site of pBR322. It contains the following restriction sites (approximate kb from the EcoRI site): BamHI ? 0.38; PvuII ? 2.1, 6.6; AccI ? 0.65, 4.5, 5.7, 6.7; HincII ? 0.65, 3.7, 8.4. The 2.2 AccI/HincII fragment is essential for replication in A. calcoaceticus.