Crosslinking, Labeling and Protein Modification
Crosslinking, Labeling and Protein Modification
Enzo Life Sciences Rhod-2 AM (ultra pure) (1mg). CAS: 129787-64-0
Rhod-2 AM has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, making it suitable for use with argon and krypton laser excitation sources. Rhod-2 signal intensity one of the strongest of all the calcium probes. Rhod 2 is especially suitable probe for intracellular calcium monitoring by confocal laser-scanning microscopy and flow cytometry. The dissociation constant of Rhod 2 with calcium is among the highest of all the fluorescent calcium probes, providing a wider range for monitoring calcium concentration. Wavelength Maxima: Excitation 549nm, Emission 578nm. Alternative name: 1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)]ethane-N,N,N',N'-tetraacetic acid, tetraacetoxy methyl ester . Br. Purity: ≥90% (HPLC). Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Biotium Fura-2, Pentapotassium Salt
Fura-2, pentaammonium salt is a ratiometric fluorescent dye that binds to free intracellular calcium. It is membrane-impermeant and may be loaded into cells via microinjection or scrape loading. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2-free) to 335 nm (Ca2-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm.Properties: Light yellow solid soluble in DMSO; Store at -20°C, protect from light, especially in solution; Kd: 145 nM (22°C, pH 7.2 buffer); Ex/Em = 363/512 nm, e = 27,000 M-1cm-1 (no Ca2); Ex/Em 335/505 nm, e = 35,000 M-1cm-1 (high Ca2); C29H22K5N3O14; MW: 832; [113694-64-7].
Advanced Biomatrix PhotoAlginate-Ink methacrylated alginate bioink for BIONOVA X DLP Printer 10 mL
PhotoAlginate is 10 mL of sterile, methacrylated alginate (2% solution with 0.5% LAP) bioink for 3D Bioprinting with the BIONOVA X. Methacrylated Alginate is a useful tool, as it can be crosslinked with light or calcium.
Advanced Biomatrix PhotoAlginate Methacrylated Alginate 200 mg sterile
PhotoAlginate is a sterile, lyophilized, methacrylated alginate. It creates 3D hydrogels that can be prepared at multiple concentrations and crosslinked using light or calcium to provide various gel stiffness. PhotoAlginate can be mixed with other photocrosslinkable ECM's.
Cayman Chemical dPE-PEG 2000-amInsodIum 100mg
An herbicide reversibly decreases H. vulgare root and shoot length and inhibits in vitro paclitaxel-induced tubulin polymerization in a dose-dependent manner completely inhibits tubulin polymerization in Hemanthus endosperm cells at a concentration of 0.1 M inhibits calcium accumulation in corn mitochondria (ID50 140 nM) and induces a 3-fold increase in the rate of calcium efflux from rat liver mitochondria at a concentration of 100 nM inhibits the growth of P. falciparum (IC50 3.5 M) completely inhibits microtubule polymerization in P. falciparum trophozoites at a concentration of 20 M
Enzo Life Sciences D-myo-Inositol 1,4,5-trisphosphate . hexapotassium salt (1mg). CAS: 103476-24-0
Product of phosphatidylinositol phospholipid hydrolysis. Causes release of calcium from intracellular stores. Alternative name: Ins(1,4,5)P3 . 6K, IP3. Purity: ≥98% (TLC). Appearance: White to off-white powder. Solubility: Soluble in water. Long Term Storage: -20°C.
Enzo Life Sciences D-myo-Inositol 1,4,5-trisphosphate . hexapotassium salt (5mg). CAS: 103476-24-0
Product of phosphatidylinositol phospholipid hydrolysis. Causes release of calcium from intracellular stores. Alternative name: Ins(1,4,5)P3 . 6K, IP3. Purity: ≥98% (TLC). Appearance: White to off-white powder. Solubility: Soluble in water. Long Term Storage: -20°C.
Cayman Chemical 6-keto LIthocholIc AcId 50mg
A positive allosteric modulator of mGluR5 potentiates glutamate-induced increases in intracellular calcium in CHO cells expressing mGluR5 (EC50s 10 and 20 nM for the human and rat receptor respectively) inhibits amphetamine-induced PPI disruptions in rats at 30 mg/kg s c
Sigma Aldrich Fine Chemicals Biosciences Collagenase from Clostridi5G
Collagenase enzyme from Clostridium histolyticum has collagen binding domain (CBD) and polycystic kidney disease-like (PKD-like) domains. It also has Ca2 binding site for calcium binding which is crucial for its functionality.nbspCollagenase is used in the management of connective tissue disorder Dupuytren contracture.
Vector Laboratories Concanavalin A (Con A) Fluorescein 25 mg
Concanavalin A (ConA) recognizes α-linked mannose residues present as part of a "core oligosaccharide" in many serum and membrane-bound glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer, but below pH 5.6, it dissociates into dimers of 52 kDa. Derivatizations can produce stable forms with dimeric structures. Con A requires calcium or manganese ions at each binding site, so avoid using buffers that bind calcium (such as phosphate) to dilute Con A since a gradual loss in activity may occur. This fluorescein-labeled Con A features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is a mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside.
Cayman Chemical TCEP-botn 1mg
An mGluR antagonist the active component of MCPG inhibits ACPD-induced intracellular calcium increases in CHO cells expressing mGluR1a (IC50 120 UM) has agonist activity at mGluR2 inducing long-term depression in rat hippocampal dentate gyrus an effect that can be blocked by an mGluR2 antagonist blocks long-term potentiation in the CA1 region
Cayman Chemical TrImethoprIm-PEG-amIntrIfl 1mg
An endogenous peptide inhibits a-MSH release from primary frog NILs (ED50 100 nM) inhibits thyrotropin-releasing hormone-induced increases in intracellular calcium in primary frog melanotrope cells at 100 nM increases food intake in frog larvae at 5 or 10 nmol/kg i.c.v. increases mean blood pressure in normotensive sharks at 5 nmol/kg
Cayman Chemical ANTIBODY Psoralen 50mg
A potent aminoalkylindole CB receptor agonist (Ki 62 3 and 3 3 nM for human recombinant CB1 and CB2 receptors respectively) increases extracellular glutamate levels in primary cultures of rat cerebral cortex neurons displaying a bell-shaped concentration-response curve induces release of the proinflammatory neuropeptide CGRP from trigeminal ganglion neurons in a calcium-dependent manner (EC50 26 UM)
Biotium Fura-2, AM Ester, 20x50ug
Fura-2, AM ester is an intracellular fluorescent calcium indicator, and the preferred dye for ratiometric imaging microscopy. The membrane-permeant AM ester can be loaded into cells via incubation, but due to limited water solubility, a mild detergent Pluronic F-127 is often used to facilitate cell loading. Fura-2, AM ester itself does not bind Ca2, but intracellular esterases readily hydrolyze it to Fura-2. Upon calcium binding, the excitation maximum of Fura-2 undergoes a blue-shift from 363 nm (no Ca2) to 335 nm (high Ca2) while the emission maximum is relatively unchanged. Measure by flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Properties: Light yellow solid soluble in DMSO; Store at -20°C, protect from light, especially in solution; Kd: 145 nM (22°C, pH 7.2 buffer); Ex/Em = 363/512 nm, e = 27,000 M-1cm-1 (no Ca2); Ex/Em 335/505 nm, e = 35,000 M-1cm-1 (high Ca2); C44H47N3O24; MW: 1001.9; [108964-32-5].
Enzo Life Sciences Dihydrorhodamine 123 (ultra pure) (10mg). CAS: 109244-58-8
Dihydrorhodamine 123 is a widely utilized probe for detection of intracellular reactive oxygen species (ROS) such as peroxide, hypochlorous acid and peroxynitrite. It is readily oxidized into rhodamine 123, which exhibits a spectral profile similar to that of FITC. In combination with other fluorescent probes (such as surface receptor-targeted fluorescent antibodies, the cell viability probe propidium iodide, or fluorescent calcium indicators) dihydrorhodamine 123 can be used for multiparametric cell measurements. Wavelength Maxima: Excitation 507nm, Emission 529nm Alternative name: 2-(3,6-Diamino-9H-xanthene-9-yl)-benzoic acid methyl ester. Purity: ≥95% (HPLC). Solubility: Soluble in DMSO. Long Term Storage: -20°C.
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