Gel Filtration Media and Columns
AdipoGen AMPAdePi
Protein. Full-length NAD 5’-nucleotidase Haemophilus influenzae without the signal peptide corresponding to the first 26 aa. Source E. coli. Endotoxin content N/A. Liquid. In 5mM KH2PO4, pH 6.8 containing 100mM TRIS-HCl pH 8.5 and 10mM MgCl2. Purity >98% SDS-PAGE. NadN NAD nucleotidase is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NADP for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR nicotinamide riboside, NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognizing either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5-adenosine monophosphate. It can be used as an economically alternative to produce NR nicotinamide riboside. Catalytic activity NAD=AMP NMN=AdeNRPPi
