Cyclin B Mouse, Unlabeled, Clone: 18, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 610220

Catalog No. BDB610220

Description

The B-type cyclins serve as subunits of the major mitotic protein kinase in many different organisms. In mammals, Cyclin B is a subunit for the cdc2 (Cdk1) protein kinase and is synthesized during late S and G2 phases. Cyclin B associates with inactive cdc2 and facilitates phosphorylation of cdc2 at Thr-14 and Tyr-15. This phosphorylation maintains the inactive state until the end of G2. The inactive Cyclin B-cdc2 complex continues to accumulate in the cytoplasm until the CDC25 phosphatase dephosphorylates Thr-14 and Tyr-15, rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B becomes degraded. This degradation process appears to be ubiquitin-dependent and is necessary for the cell to exit mitosis. Also, the conserved domain in B-type cyclins, known as the P box, is required for CDC25 activation.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: Cyclin B
• Classification: Monoclonal
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Quantity: 150 μg
• Research Discipline: Cell Biology
• Target Species: Human, Rat
• Isotype: IgG1

• Applications: Western Blot
• Clone: 18
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.