Applied Biosystems™ SYBR™ Green PCR Master Mix

Everything needed for SYBR™ Green dye–based PCR amplification and detection in a convenient, single-tube format.

Supplier:  Applied Biosystems™ 4344463

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Catalog No. 43-444-63

Includes: 2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components

Description

Applied Biosystems™ SYBR™ Green PCR Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

• Premixed components stored at 2° to 8°C significantly reduce assay setup time
• SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability

Maximum Flexibility and Convenience:

• Applied Biosystems™ SYBR™ Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
• SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
• The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
• Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
• This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
• SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.

  Order Info

  Shipping Condition: Wet Ice

Specifications

• Content And Storage: SYBR™ Green PCR Master Mix in a 1 mL vial. Sufficient reagents provided for 40 reactions based on a 50 μl reaction volume. Store at -20°C.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Form: Concentrated
• GC-Rich PCR Performance: High
• Polymerase: AmpliTaq Gold DNA Polymerase
• Technique: Fluorescence Intensity
• Label or Dye: SYBR Green
• Product Line: SYBR™
• Quantity: 1 x 1 mL
• Sufficient For: 40 Reactions of 50 μL
• For Use With (Application): Gene Expression
• Hot Start: Built-In Hot Start
• Sample Type: DNA

• Detection Method: SYBR
• Format: Tube
• PCR Method: qPCR
• Reaction Speed: Standard
• For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, GeneAmp™ 5700
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: Real Time PCR SYBR Master Mix
• Shipping Condition: Wet Ice
• Target Specificity: Not Target-Specific
• Concentration: 2X
• No. of Reactions: 40 Reactions
• Volume: 1 x 1 mL

For Research Use Only. Not for use in diagnostic procedures.