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Applied Biosystems™ SYBR™ Green PCR Master Mix
SDP

Everything needed for SYBR™ Green dye–based PCR amplification and detection in a convenient, single-tube format.

Supplier:  Applied Biosystems™ 4344463

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Catalog No. 43-444-63

  • $169.65 / Each of 1

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    Reg : $178.00

    The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
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Includes: 2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components

Description

Description

Applied Biosystems™ SYBR™ Green PCR Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

  • Premixed components stored at 2° to 8°C significantly reduce assay setup time
  • SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
  • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
  • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
  • Proprietary buffer enhancements ensure performance and reliability

Maximum Flexibility and Convenience:
  • Applied Biosystems™ SYBR™ Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
  • SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
  • The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
  • Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
  • This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
  • SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.

    Order Info

    Shipping Condition: Wet Ice

Specifications

Specifications

SYBR™ Green PCR Master Mix in a 1 mL vial. Sufficient reagents provided for 40 reactions based on a 50 μl reaction volume. Store at -20°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Concentrated
High
AmpliTaq Gold DNA Polymerase
Fluorescence Intensity
SYBR Green
SYBR™
1 x 1 mL
40 Reactions of 50 μL
Gene Expression
Built-In Hot Start
DNA
SYBR
Tube
qPCR
Standard
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, GeneAmp™ 5700
ROX (Pre-mixed)
Real Time PCR SYBR Master Mix
Wet Ice
Not Target-Specific
2X
40 Reactions
1 x 1 mL
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For Research Use Only. Not for use in diagnostic procedures.