Real Time PCR Master Mixes

Real Time PCR Master Mixes
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Fisherbrand™ SYBR™ Green qPCR Master Mix
Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Content And Storage | SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark |
Product Type | Master Mix |
Form | Frozen |
Reaction Speed | Fast or Standard |
Label or Dye | SYBR Green,FAM |
Concentration | 2X |
For Use With (Application) | Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR) |
PCR Method | qPCR,2-step RT-qPCR,RT-qPCR |
Detection Method | SYBR |
Applied Biosystems™ Fast SYBR™ Green Master Mix
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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GC-Rich PCR Performance | High |
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Purity or Quality Grade | UP (Ultra Pure) |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast |
Label or Dye | SYBR Green |
Product Line | SYBR™ |
Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0
Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Purity or Quality Grade | UP (Ultra Pure) |
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Concentration | 2X |
For Use With (Application) | Gene Expression,Genotyping,Pathogen Detection,Virus Detection |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Bottle |
Product Type | qPCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode |
Sample Type | Environmental Sample, Food |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Product Line | TaqMan™ |
Applied Biosystems™ TaqMan™ Multiplex Master Mix
Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Polymerase | AmpliTaq Fast DNA Polymerase |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Product Type | Multiplex Master Mix |
Sample Type | DNA |
Reaction Speed | Fast |
Concentration | 2X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | Multiplex qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ Absolute Q™ 1-step RT-dPCR Master Mix (4X)
Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Wet Ice |
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Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | PowerUP™, SYBR™ |
Detection Method | SYBR |
Applied Biosystems™ TaqPath™ ProAmp™ Master Mix
TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Wet Ice |
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Polymerase | Taq DNA Polymerase |
Content And Storage | Store at 2°C to 8°C in the dark. |
Product Type | qPCR Master Mix |
For Use With (Equipment) | 7500 Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, StepOne™ System |
Reaction Speed | Fast or Standard |
For Use With (Application) | Genotyping |
PCR Method | qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)
TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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Format | Tube |
For Use With (Equipment) | QuantStudio™ 6 Pro or QuantStudio™ 7 Pro, QuantStudio™ 3 or 5, QuantStudio™ 6/QuantStudio™ 7 Flex, QuantStudio™ 12K Flex, QuantStudio™ Dx, ViiA™ 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier |
Sample Type | DNA, RNA |
Reaction Speed | Fast or Standard |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR,Multiplex qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqPath™ ProAmp™ Multiplex Master Mix
TaqPath ProAmp Multiplex Master Mix is for higher multiplexing (up to four targets) in high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Wet Ice |
---|---|
Polymerase | Taq DNA Polymerase |
Content And Storage | Store at 2°C to 8°C in the dark. |
Product Type | qPCR Master Mix |
For Use With (Equipment) | QuantStudio™ 3 or 5, QuantStudio™ 6/QuantStudio™ 7 Flex, QuantStudio™ 12K Flex, QuantStudio™ Dx, ViiA™ 7 Real-Time PCR System ViiA™ 7 Dx, 7500 Fast, StepOne™ or StepOnePlus™ |
Reaction Speed | Fast or Standard |
For Use With (Application) | Genotyping |
PCR Method | Multiplex qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG
Is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2).


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Product Type | Quantitative PCR SuperMix-UDG |
For Use With (Equipment) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | Platinum™ |
Detection Method | Primer-probe |
Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG w/ROX
qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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Polymerase | Platinum Taq DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Product Type | Quantitative PCR SuperMix-UDG |
For Use With (Equipment) | 7000 System, 7300 System, 7700 System, 7900HT System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | Platinum™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Genotyping Master Mix
Optimized for end-point fluorescence detection in SNP genotyping applications.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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GC-Rich PCR Performance | High |
---|---|
Fidelity (vs. Taq) | 2 X |
Purity or Quality Grade | UP (Ultra Pure) |
Concentration | 2X |
For Use With (Application) | Genotyping |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Genotyping Target | Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known) |
Product Type | Genotyping Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 |
Sample Type | DNA (Genomic) |
Reaction Speed | Standard |
Technique | SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation) |
Product Line | TaqMan™ |
Applied Biosystems™ Path-ID™ qPCR Master Mix
Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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Polymerase | AmpliTaq Gold DNA Polymerase |
Purity or Quality Grade | UP (Ultra Pure) |
Format | Tube |
Passive Reference Dye | ROX (Pre-mixed) |
Form | Liquid |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
For Use With (Application) | Animal Health,Pathogen Detection |
PCR Method | qPCR |
Product Line | Ambion™, Path-ID™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR even in the presence of challenging PCR inhibitors.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
Reverse Transcriptase | M-MLV |
Form | Liquid |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Format | Tube |
Product Type | 1-Step Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | Total RNA, Viral DNA, Viral RNA, mRNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Product Line | TaqMan™ |
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Multiplex Master Mix for qPCR (No ROX)
TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) is designed to enable dye flexibility for multiplexing up to four different RNA/DNA targets for high-throughput RT-qPCR.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
Passive Reference Dye | None |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | RNA, DNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Concentration | 4X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR,Multiplex qPCR |
Detection Method | Primer-probe |