CD5 Monoclonal Antibody (UCHT2), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
14-005-995	LIFE TECHNOLOGIES 14005995	10 mg
14-005-937	LIFE TECHNOLOGIES 14005937	2 mg

Description

The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation. The monoclonal antibody UCHT2 recognizes primate CD5. Applications Reported: The UCHT2 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. Applications Tested: The UCHT2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD5 Monoclonal antibody specifically detects CD5 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry (Frozen), Western Blot

Specifications

• Antigen: CD5
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: CD antigen CD5; CD5; CD5 antigen; CD5 antigen (p56-62); CD5 antigen p56-62; Cd5 molecule; CD5 protein; CD74 antigen, invariant polypeptide of major; fCD5; LEU1; Ly-1; Ly-12; Ly-A; Lymphocyte antigen 1; Lymphocyte antigen CD5; lymphocyte antigen T1/Leu-1; Lyt-1; Pan T cell; T1; T-cell surface glycoprotein CD5; unnamed protein product
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: UCHT2
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P06127
• Gene ID (Entrez): 921
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: Cd5