Novus Biologicals™ DAPI Solution

Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Immunohistochemistry Whole-Mount, Immunofluorescence, Immunocytochemistry

• DAPI is excluded by viable cells but can penetrate cell membranes of dying or dead cells, in which it intercalates into double stranded nucleic acids.
• Dead cells which take up DAPI will fluoresce brightly around 461nm.
• DAPI may be excited by the UV or violet lasers although the UV laser is much more efficient.
• DAPI's emission spectra is very similar to the Pacific Blue (TM) dye.
• Alternate names for DAPI Solution:
  • 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride
  • 4,6-Diamidino-2-phenylindole dihydrochloride
  • DAPI dihydrochloride
• Dilutions: Immunocytochemistry/Immunofluorescence 0.1-1.0μg/mL

Quantity

1 mg

Content And Storage

Store at -20°C in the dark. Avoid freeze-thaw cycles.