Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

Description

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

• Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
• Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
• Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
• Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

• Average particle size: 50μm
• Matrix: Allyl dextran and N, N'-methylenebisacrylamide
• pH Stability working range: 3 to 11
• pH Stability cleaning range: 2 to 13
• Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
• Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm

Column

• Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

• Media:
• Fractionation [Mr] globular proteins: 2 x 104^-8 x 106
• Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
• Fractionation range [Mr] DNA exclusion limit:    271bp
• Fractionation [Mr] Dextrans: 1 x 104^-2 x 106

HiPrep 16/60 Sephacryl S-500 HR

• Media:
• Exclusion limit for Dextrans: 100 x 106
• Fractionation range [Mr] DNA exclusion limit: 1078bp
• Fractionation [Mr] Dextrans: 4 x 104^-2 x 107

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Specifications

• Quantity: Each
• Flow Rate: <1.3 mL/min.
• Particle Size: 25 to 75 μm
• For Use With (Equipment): Low to medium pressure AKTA™ systems or pumped chromatography systems
• Bed Volume: 120 mL
• Product Type: Sephacryl HR Pre-packed Gel Filtration Column
• Diameter (Metric) Inner: 1.6cm
• For Use With (Application): Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
• Height (English): 23.62 in.
• Height (Metric): 60cm
• Material: Polypropylene
• Max. Pressure: 1.5 bar [0.15 MPa] (22 psi)
• pH Range: 3 to 11
• Support Type: Allyl Dextran and N,N'-methylenebisacrylamide
• Sample Type: Polysaccharides, macromolecules with extended structures and even small plasmids
• Content And Storage: 4°C to 30°C, 20% Ethanol