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Gel Filtration Media and Columns

Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1 – 15 of 106 results
1

Cytiva Sephadex™ LH-20
GSA_VA

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

EDGE
Particle Size 18 to 111 μm in dry form
Height (Metric) 30cm
Flow Rate 720 cm/hr.
Content And Storage 4 to 30°C
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex LH-20 Media
Sample Type Steroid, Terpenoid, Lipid, low molecular weight Peptide
Diameter (Metric) Inner 2.6cm
For Use With (Application) For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
Support Type Hydroxypropylated, cross-linked Dextran
Max. Pressure 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
Height (English) 11.81 in.
2

Cytiva HiTrap™ Heparin HP Columns
GSA_VA

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

EDGE
Particle Size 34 μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 5 to 10
Product Type Heparin HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
3

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns
GSA_VA

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

EDGE
4

Cytiva HiTrap™ NHS-Activated HP Columns
GSA_VA

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

EDGE
Particle Size 34 μm
Ligand Type 10μM NHS groups/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 8°C
pH Range 3 to 12
Product Type NHS-Activated HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
5

Cytiva HiTrap™ Capto™ Core 400

Designed for intermediate purification and polishing of viruses and other large biomolecules

6

Cytiva Sephadex™ G15 Media

Gel filtration medium for desalting and buffer exchange for small biomolecules greater than 1500 molecular weight

7

Cytiva Superose™ Media Prep Grade Lab Packs

High performance gel filtration media for scaleup to preparative purifications of biomolecules

Particle Size 30 to 40 μm
Flow Rate 40 cm/hr.
Bed Volume ≈100 mL
pH Range 3 to 12
For Use With (Equipment) AKTA™ design pump or system
Sample Type Proteins, Peptides, Polynucleotides, Wide Variety of Biomolecules
Quantity Each
For Use With (Application) Separations of Proteins and other biomolecules according to size
Support Type Highly Cross-linked Agarose
8

Cytiva Sephadex™ G-50 and G-100 DNA Grade Agarose Gels
GSA_VA

Used for purification of DNA from small molecules. Cytiva illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration.

Content And Storage 4° to 30°C
Format Beaded Gel
pH Range 2 to 10
Product Type G-50 and G-100 DNA Grade Agarose Gels
For Use With (Equipment) Auto Seq G-50 column, ProbeQuant G-50 micro column, MicroSpin G-50 column and NICK column
Sample Type DNA
Support Type Cross-linked Dextran with Epichlorohydrin
9

Cytiva HiPrep™ Sephacryl™ S-200 HR Columns
GSA_VA

Maximum recovery and minimum nonspecific adsorption

Particle Size 25 to 75 μm
Height (Metric) 60cm
Content And Storage 4°C to 30°C, 20% Ethanol
pH Range 3 to 11
Product Type Sephacryl HR Column
For Use With (Equipment) Low to medium pressure AKTA™ systems
Sample Type Antibodies, serum proteins and mid-size proteins
Quantity Each
Material Polypropylene
For Use With (Application) Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
10

Cytiva HiTrap™ Blue HP Columns

For purifying albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons and related proteins

Particle Size 34 μm
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 4 to 12
Product Type Blue HP Columns
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type Albumin, Interferon, Nucleotide-dependent Enzymes, a2-macroglobulin, Coagulation Factors and Related Proteins
Material Polypropylene
For Use With (Application) Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
11

Cytiva HiTrap™ Benzamidine FF (high sub) Columns

Remove or purify serine proteases such as thrombin. Cytiva Life Sciences™ HiTrap™ Benzamidine FF (high sub) Columns are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub).

Particle Size 90 μm
Ligand Type >12μM p-aminobenzamidine/mL medium
Height (Metric) 2.5cm
Content And Storage 2° to 8°C
pH Range 2 to 8
Product Type Benzamidine FF Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Sample Type Serine Proteases such as Thrombin
Material Polypropylene
For Use With (Application) Removal or purification of Serine Proteases such as Thrombin
Support Type 4% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
12

Cytiva HiTrap™ Streptavidin HP Column
GSA_VA

Use for fast and easy preparative purifications either alone or connected in series. HiTrap™ Streptavidin HP Column are convenient and reliable prepacked columns.

Promotions are available
13

Cytiva Superdex 30 Increase Precision 3.2/300 GL Prepacked SEC Column

Designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500μL

14

Cytiva Superdex™ 200 Prep Grade
GSA_VA

Produce separation medium with high selectivity and resolution. Cytiva Life Sciences™ Superdex™ 200 Prep Grade for purifying proteins of fractionation range: 10000 to 600000D

15

Cytiva Sephacryl™ S-500 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Height (Metric) 15cm
Content And Storage 4° to 30°C
Bed Volume 19 to 230L
pH Range 3 to 11
Diameter (Metric) Inner 40 to 140cm
For Use With (Application) Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale
Max. Pressure 100kPa
Particle Size 25 to 75 μm
Flow Rate >125 cm/hr.
Format Solid Spherical Bead
Product Type Sephacryl HR Media
Sample Type Polysaccharides, macromolecules and small plasmids
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Height (English) 5.9 in.