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Molecular Probes™ BacLight™ RedoxSensor™ Green Vitality Kit
SDP

Catalog No. B34954
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1 kit

BacLight™ RedoxSensor™ Green Vitality Kit

The RedoxSensor™ Green reagent included in the BacLight™ RedoxSensor™ Green Vitality Kit is an indicator of bacterial reductase activity. This reductase activity is, in turn, a reliable marker for changes in electron transport chain function and for changes in vitality that occurs following antibiotic treatment. RedoxSensor™ Green reagent penetrates both gram-positive and gram-negative bacteria. Following reduction, the RedoxSensor™ Green reagent will produce a stable green-fluorescent signal in 10 minutes that is compatible with formaldehyde fixation techniques.

Specifications

Cell Type Bacteria
Content And Storage Contains 1 vial of RedoxSensor™ green (200 μL, 1 mM solution in DMSO), 1 vial of propidium iodide (300 μL, 20 mM solution in DMSO), 1 vial of sodium azide (1 mL of a 2 M solution in water), and 1 vial of CCCP (400 μL, 5mM in DMSO).
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Green Vitality Kit
Dye Type RedoxSensor™ green, PI
Emission PI: 490⁄635, RedoxSensor™ green: 490⁄520
Form Solution
Format Tube(s), Slide(s)
Product Line BacLight™, RedoxSensor™
Quantity 200
Shipping Condition Room Temperature
Solubility DMSO (Dimethylsulfoxide)
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For Research Use Only. Not for use in diagnostic procedures.

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