Cell Function Reagents and Kits
Cell Function Reagents and Kits
Invitrogen™ eBioscience™ Propidium Iodide
Standard reagent used for assessing cell viability and exclusion of non-viable cells in flow cytometry. PI binds to double-stranded DNA, but is excluded from cells with intact plasma membranes.
Invitrogen™ eBioscience™ Propidium Iodide Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Propidium Iodide binds to double-stranded DNA by intercalating between basepairs, but is excluded from cells with intact plasma membranes.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 506/780 Sample Pack
Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.
Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.
Invitrogen™ eBioscience™ CFSE
Widely used for cell tracking and proliferation studies. It has also been used in CTL assays and cell motility studies. CFSE readily crosses intact cell membranes.
Invitrogen™ eBioscience™ Calcein Violet 450 AM Viability Dye
Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Violet fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Violet 450.
Invitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 450
A violet fluorescent dye that can be used to monitor individual cell divisions for up to 7 generations.
Invitrogen™ Newport Green™ DCF Diacetate, cell permeant
Has moderate zinc-binding affinity (Kd for Zn2+ approximately 1μM) but is essentially insensitive to Ca2+
Invitrogen™ NP-EGTA (o-Nitrophenyl EGTA, Tetrapotassium Salt, cell impermeant
Photolabile chelator that exhibits high selectivity for Ca2+
Thermo Scientific™ Hypoxia Green Reagent for Flow Cytometry
Hypoxia Green Reagent for Flow Cytometry is an antibody-independent reagent used to detect low levels of oxygen levels in live cells.
Invitrogen™ Image-iT™ Green Hypoxia Reagent
Image-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells.
Molecular Probes™ LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit, for flow cytometry
The LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit allows researchers to reliably distinguish and quantitate live and dead bacteria with the aid of a flow cytometer, even in a mixed population containing a range of bacterial types.
Invitrogen™ LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation
Determines the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation.