Invitrogen™ eBioscience™ Indo-1 AM Calcium Sensor Dye

Description

Membrane-permeable dye used to determine changes in calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Once Indo-1 enters the cell, esterases cleave the AM group yielding a membrane-impermeable dye.

• Unbound Indo-1 has a peak emission at 475nm
• Upon binding calcium, peak emission shifts down to 410nm
• In flow cytometry this can be measured over time and can be represented as a ratio of the two emission wavelengths
• To optimize the ratio between the two emissions, unbound Indo-1 fluorescence should be collected using a filter above 485nm; bound Indo-1 fluorescence should be collected using a filter below 400nm
• Because the emission profile of Indo-1 is broad, multicolor flow analysis using eFluor ™ 450, eVolve™ 605 or eVolve™ 655 is not possible, however fluorochrome conjugated antibodies utilizing the 488nm or 633 nm are quite compatible with Indo-1

• Molecular weight: 1009.91g/mol
• Peak excitation: 346nm
• Peak emission: 475nm
• Reconstitute in high-quality, freshly opened DMSO
• Recommended concentration: 1 mM
• Once reconstituted, store at −20°C with dessicant and use within a short period of time. Avoid freeze-thawing

Specifications

• Color: Blue
• Detection Method: Fluorescence
• For Use With (Application): Immunohistochemistry (IHC), Flow Cytometry
• For Use With (Equipment): Fluorescence Microscope
• Product Type: Dye
• Emission: 346/475
• Product Line: eBioscience
• Quantity: 1 mg

For Research Use Only. Not for use in diagnostic procedures.