Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

Supplier:  Invitrogen™ K250020

Catalog No. K250020

Includes: TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.

Description

• Contains minimal MCS, so sequence more of insert and less of vector
• Patented att sites, pCR™8 vector to create entry clone, which facilitates easy access via Gateway recombination cloning to variety of expression vectors without tedious sub-cloning, saving valuable research time
• att sites for rapid recombination into a variety of Gateway destination vectors
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site
• Spectinomycin resistance gene for robust selection in E. coli
• EcoRI sites flanking the PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

Specifications

• Cloning Method: TOPO™ with Gateway™
• No. of Reactions: 20 reactions
• Product Type: TA Cloning Kit

• Content And Storage: The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• Product Line: One Shot™
• Vector: pCR

For Research Use Only. Not for use in diagnostic procedures.