Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.
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Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
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Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
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Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System
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Approved for shipment at Room Temperature or on Wet or Dry Ice
Promoter
T3, T7
Content And Storage
Box 1: Store at -5 to -30°C. • pCR XL-2-TOPO vector • Salt Solution • T3 Primer • T7 Primer • XL Control PCR Template • XL Control PCR Primers • Platinum SuperFi Green PCR Master Mix • SuperFi GC Enhancer • Nuclease-free water
Box 2: Store at room temperature. • Gel Solubilization Buffer (L3) • Binding Buffer (B2) • Wash Buffer (W1) • Elution Buffer (E1) • PureLink Clean-up Spin Columns (in Wash Tubes) • PureLink Recovery/Elution Tubes
Box 3: • One Shot OmniMAX 2 T1R chemically competent E. coli • S.O.C. Medium • pUC 19 Control DNA
The DNA contained in the preparation has been lyophilized to maximize stability.The two hydrolytic enzymes used in this kit, shrimp alkaline phosphatase and exonuclease I, effectively remove excess dNTPs and primers.
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This is a Capillary Array for the Applied Biosystems™ 3730 DNA Analyzer. Each array contains 48 capillaries, 36cm long. Used for both sequencing and fragment analysis runs.
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This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.
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