Thermo Scientific™ beta-Galactosidase Assay Reagent

Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.

Catalog Number	Mfr. No.	Quantity
PI75705	Thermo Scientific™ 75705	25 mL
PI75710	Thermo Scientific™ 75710	3 x 25 mL

Description

Perform a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity using Thermo Scientific™ Mammalian β-Galactosidase Assay Kit.

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Highlights:

• Choose stand-alone assay reagent or complete kit for cell lysis and detection
• Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
• Adaptable to 96-well microplates or tube assay formats
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatiible with prepared lysates from other species such as bacteria

Includes:

Stand-alone Thermo Scientific Pierce™ β-Galactosidase Assay Reagent, Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Part No. 78503), and ready-to-use Stop Solution

Specifications

• Assay: Reporter Enzyme
• Detection Method: Colorimetric
• For Use With (Application): Absorbance
• Substrate: Beta-Gal Substrate
• Target Enzyme: Beta-Galactosidase
• For Use With (Equipment): Microplate Reader
• Product Type: Pierce beta-Galactosidase Assay Reagent

• Content And Storage: Store at -20°C.
• Form: Liquid
• Product Line: Pierce™
• Substrate Properties: Chemical Substrate
• Format: 96-well Plate
• Label or Dye: Enzyme Labeled

For Research Use Only. Not for use in diagnostic procedures.