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Substrates

Substrates

Substrates are laboratory reagents, chemicals, and consumables, and kits used to support or enhance biomedical, pharmaceutical, clinical, food science, environmental, and other research and testing laboratory processes. Products include enzymes, inhibitors, acids, bases, buffers, antibodies, and chromatography supplies.
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1 – 15 of 1,077 results
1

Thermo Scientific™ IPTG

Detect beta-galactosidase expression from vectors, plasmids, or DNA fragments with this chromogenic substrate.

EDGE
2

Novex™ AP Chemiluminescent Substrate (CDP-Star™)

Novex™ AP Chemiluminescent Substrate (CDP-Star™)

EDGE ENCOMPASS_PREFERRED
3

Invitrogen™ MUP (4-Methylumbelliferyl Phosphate, Free Acid)

MUP is a fluorescent substrate for alkaline phosphatases

EDGE ENCOMPASS_PREFERRED
4

Thermo Scientific™ beta-Galactosidase Assay Reagent

Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.

EDGE
Substrate Beta-Gal Substrate
Assay Reporter Enzyme
Substrate Properties Chemical Substrate
Content And Storage Store at -20°C.
Format 96-well Plate
Product Type Pierce beta-Galactosidase Assay Reagent
For Use With (Equipment) Microplate Reader
Form Liquid
Label or Dye Enzyme Labeled
Target Enzyme Beta-Galactosidase
For Use With (Application) Absorbance
Product Line Pierce™
Detection Method Colorimetric
5

Invitrogen™ Rhodamine 110, bis-(p-Tosyl-L-Glycyl-L-Prolyl-L-Arginine Amide)

This Molecular Probes™ bisamide derivative of rhodamine 110 (R110) is a sensitive and selective substrate for assaying proteases in solution or inside living cells.

EDGE ENCOMPASS_PREFERRED
6

Invitrogen™ Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate)

Measure results in microplate assays or using flow cytometry

EDGE ENCOMPASS_PREFERRED
7

Invitrogen™ PI(3,4)P2 Lipid Kinase Substrate

The PI(3,4)P2 Lipid Kinase Substrate has been specifically optimized for use with Type I PIP kinases (e.g., PIP5K1A, PIP5K1B, PIP5K1C)

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Substrate Phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2)
Content And Storage Phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2) in 30 mM HEPES pH 7.5 buffer.
Form Powder
Substrate Type Kinase Substrate
Product Line Invitrogen™
8

Invitrogen™ DiFMUP (6,8-Difluoro-4-Methylumbelliferyl Phosphate)

Continuously assay acid phosphatases at low pH

EDGE ENCOMPASS_PREFERRED
Shipping Condition Room Temperature
Excitation/Emission 358⁄455
Substrate Phosphatase Substrate
Substrate Properties Chemical Substrate
Content And Storage Store in freezer (-5 to -30°C).
For Use With (Equipment) Microplate Reader
Form Powder
Label or Dye DiFMU
Color Blue
Target Enzyme Phosphatases
Detection Method Fluorescence
9

Invitrogen™ CMAC, t-BOC-Leu-Met (7-Amino-4-Chloromethylcoumarin, t-BOC-L-Leucyl-L-Methionine amide)

Cleavage produces blue-fluorescence

EDGE ENCOMPASS_PREFERRED
10

Invitrogen™ Fluorescein Di-β-D-Glucuronide (FDGlcU)

Cell permeant

EDGE ENCOMPASS_PREFERRED
11

Invitrogen™ Resorufin β-D-Galactopyranoside

For the hydrolysis product, resorufin

EDGE ENCOMPASS_PREFERRED
12

Novex™ AP Chromogenic Substrate (BCIP/ NBT)

Novex™ AP Chromogenic Substrate (BCIP/ NBT)

EDGE ENCOMPASS_PREFERRED
13

Gibco™ CELLstart™ Substrate

CELLstart™ Substrate

ENCOMPASS_PREFERRED
14

BD AEC Substrate Kit

Substrate for peroxidase and is a widely used chromogen for immunohistochemical staining and immunoblotting

pH 7.2
Substrate Type AEC Substrate
Storage Requirements 4°C
For Use With (Application) Immunohistochemical staining and immunoblotting, ELISPOT
Chromagen Type AEC
15

Invitrogen™ Alexa Fluor™ 647-Poly-GT Peptide Substrate

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Excitation/Emission 340/665 nm
Substrate Random (Glu:Tyr) polymer in a ratio of 4:1
Substrate Properties Peptide-Based Substrate
Form Liquid
Label or Dye Alexa Fluor™ 647
Color Far-Red
Target Enzyme Tyrosine Kinases
Substrate Type Kinase Substrate
Validated Kinases EGFR
Technique TR-FRET
Detection Method Fluorescence