Cytiva Sephacryl™ S-500 High Resolution Media

Description

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Chromatography media offer good recovery and low nonspecific adsorption. Enable gel filtration at laboratory and industrial scale, and may be purchased in prepacked columns.

• For separating polysaccharides, macromolecules with extended structures, and small plasmids
• Excellent purification over a wide molecular weight range
• High reproducibility due to long-term chemical and physical stability
• High flow rates and recoveries
• Well-suited to industrial-scale use
• Storage conditions: 20% ethanol; 4° to 30°C
• Available in a variety of selectivities and volumes

Chemical Stability (stable in all common buffers):

• 1M acetic acid, 8M urea
• 6M guanidine-HCl, 1% SDS
• 2M NaCl, 24% ethanol
• 30% propanol, 30% acetonitrile
• 0.5M NaOH (for cleaning)

pH Stability

• 3 to 11 (long-term and working)
• 2 to 13 (short-term)

Physical Stability:

• Negligible volume variation due to changes in pH or ionic strength
• Autoclavability: At 121°C, pH 7 for 30 minutes (gel only)
• Antimicrobial agent: 20% ethanol

Specifications

• Quantity: 150 mL
• Flow Rate: >125 cm/hr.
• Particle Size: 25 to 75 μm
• Bed Volume: 19 to 230L
• Product Type: Sephacryl HR Media
• Format: Solid Spherical Bead
• Diameter (Metric) Inner: 40 to 140cm
• For Use With (Application): Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale
• Height (English): 5.9 in.
• Height (Metric): 15cm
• Max. Pressure: 100kPa
• pH Range: 3 to 11
• Support Type: Allyl Dextran and N, N'-methylenebisacrylamide
• Sample Type: Polysaccharides, macromolecules and small plasmids
• Content And Storage: 4° to 30°C