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General Western Blot Reagents

General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1 – 15 of 227 results
1

Invitrogen™ Tris-Glycine Transfer Buffer (25X)

Made with high-purity reagents and are strictly quality controlled

EDGE ENCOMPASS_PREFERRED
2

Invitrogen™ iBind™ Flex Fluorescent Detection (FD) Solution Kit

Detection kit for use with iBind™ Flex Western System

EDGE ENCOMPASS_PREFERRED
3

Invitrogen™ No-Stain™ Protein Labeling Reagent

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type Protein Labeling Reagent
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility PVDF, Nitrocellulose
Shelf Life At least 2 years from receipt
Run Time 10 minutes
For Use With (Application) Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Product Line Invitrogen™
4

Thermo Scientific™ TMB Substrate Solution

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)

EDGE
5

Invitrogen™ XCell II™ Blot Module

Easy transfer of proteins or nucleic acids from mini-gels to membranes

EDGE ENCOMPASS_PREFERRED
6

Thermo Scientific™ Pierce™ Western Blot Signal Enhancer

Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.

EDGE
7

Invitrogen™ iBind™ Flex Solution Kit

Detection kit for use with iBind™ Flex Western System

EDGE ENCOMPASS_PREFERRED
No. of Reactions 10 midi blots or 20 mini blots
Product Type Flex Solution Kit
For Use With (Equipment) iBind Flex Western Device
Membrane Compatibility PVDF, Nitrocellulose
Product Line iBind™
Detection Method Colorimetric, Chromogenic, Chemiluminescence
8

Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)

Optimized, easy-to-use blocker and primary antibody diluent system

EDGE ENCOMPASS_PREFERRED
9

Invitrogen™ Incubation Tray, 10 x 14 cm

Enables convenient and even staining of E-PAGE™ blots

EDGE ENCOMPASS_PREFERRED
10

Thermo Scientific™ Restore™ Western Blot Stripping Buffer
GSA_VA

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

EDGE
Content And Storage Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature.
Form Liquid
Product Line Restore™
11

Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

EDGE
Content And Storage Upon receipt store solutions at 4°C Products are shipped at ambient temperature.
Form Liquid
Product Type Western Blot Enhancer
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal™
Detection Method Chemiluminescence, Fluorescence, Colorimetric
12

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
GSA_VA

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

EDGE
Content And Storage Upon receipt store at room temperature. Product shipped at ambient temperature.
Form Liquid
Product Line Restore™
13

Invitrogen™ Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer

Use with CSPD™ or CDP-Star™ chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF

EDGE ENCOMPASS_PREFERRED
14

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-mouse

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

EDGE ENCOMPASS_PREFERRED
15

MilliporeSigma™ ReBlot Plus Kit and Reagents
GSA_VA

Blots can be stripped of antibodies in approximately 15 minutes at room temperature

EDGE
For Use With (Application) Western Blot