Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.

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Invitrogen™ TaqMan™ MicroRNA Cells-to-C_T™ Kit

First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.

Pricing & Availability

Invitrogen™ Power SYBR™ Green Cells-to-C_T™ Kit

Ambion™ Power SYBR™ Green Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Purity or Quality Grade	LD (Low DNA)
Reverse Transcriptase	M-MLV
Purification Time	5 min.
Green Features	Fewer resources used and less waste, Less hazardous
Concentration	20X
For Use With (Application)	RT-PCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Cells
Reaction Speed	Standard
Label or Dye	SYBR Green
Hot Start	Built-In Hot Start
Product Line	Cells-to-CT™, SYBR™

Invitrogen™ Single Cell Lysis Kit

Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.

Pricing & Availability

Invitrogen™ Cells-to-cDNA™ II Kit

The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)₁₈ Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature.
Reverse Transcriptase	M-MLV
Size (Final Product)	7 kb or less
Final Product Type	cDNA
Sample Type	Mammalian Cells
Purification Time	2 hr.
Optimal Reaction Temperature	42°C
For Use With (Application)	Real Time PCR (qPCR),RT-PCR
Product Line	Ambion™, Cells-to-cDNA™

Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

Pricing & Availability

Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

Pricing & Availability

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	Tube
Product Type	1-Step qPCR kit
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Green Features	Fewer resources used and less waste, less hazardous
Starting Material	10-100,000 Cells
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	Cells-to-CT™, Power SYBR™ Green
Detection Method	SYBR

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	Tube
Product Type	1-Step qPCR kit
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Green Features	Fewer resources used and less waste, Less hazardous
Starting Material	10-100,000 Cells
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	Cells-to-CT™, TaqMan™
Detection Method	TaqMan

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
GC-Rich PCR Performance	Medium
Includes Label or Dye	Yes
Overhang	Blunt
Concentration	2X
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR
Polymerase	Phire Hot Start II DNA Polymerase
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Tissue
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Starting Material	DNA

Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit

The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2X
Green Features	Fewer resources used and less waste, Less hazardous
Concentration	2X
For Use With (Application)	Gene Expression
High-throughput Compatibility	High-throughput Compatible
PCR Method	2-step RT-qPCR
Detection Method	SYBR
Format	Tube
Product Type	Fast Advanced Cells-to-Ct Kit
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Purification Target	Lysate
Starting Material	10-100,000 Cells
Product Line	Cells-to-CT™

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2X
Green Features	Fewer resources used, less waste, less hazardous
Concentration	2X
For Use With (Application)	Gene Expression
High-throughput Compatibility	High-throughput Compatible
PCR Method	2-step RT-qPCR
Detection Method	TaqMan
Format	Tube
Product Type	Fast Advanced Cells-to-Ct Kit
Sample Type	Mammalian cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Purification Target	Lysate
Starting Material	10-100,000 Cells
Product Line	Cells-to-CT™

Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Target	DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage	The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. Components: • ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20) • ChargeSwitch™ Purification Buffer (N5) • ChargeSwitch™ Wash Buffer (W12) • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Format	Kit
Product Type	PCR Clean-Up Kit
Sample Type	PCR Products
Isolation Technology	ChargeSwitch™ Chemistry
Run Time	5 min
For Use With (Application)	Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility	High-throughput Compatible
Product Line	ChargeSwitch™

Invitrogen™ PureLink™ PCR Purification Binding Buffer B2

PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit.

Pricing & Availability

Invitrogen™ PureLink™ PCR Micro Kit

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Separation Range	100 bp to 12 kb
Content And Storage	Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.
Starting Material Volume	125 μL
Isolation Technology	Silica Spin Column
Elution Volume Max	20 μL
For Use With (Application)	Cloning,Standard PCR
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Target	DNA from Enzymatic Reactions (e.g. PCR)
Format	Spin Column
Product Type	PCR Micro Kit
Run Time	10 min.
Elution Volume Min	5 μL
Product Line	PureLink™

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance	Medium
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Plant
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
Starting Material	DNA
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR

Direct PCR Reagents and Kits

Direct PCR Reagents and Kits

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