Improve detection sensitivity in fibroblasts and whole blood.While resequencing highly expressed transcripts contributes minimally to biologically relevant information, these transcripts dominate sequencing coverage, hindering detection of low-expressing genes, rare variants, and unique fusion events associated with diseases.DepleteX utilizes Cas9 and a guide RNA set to selectively remove 70% of the most highly expressed transcripts from poly-A selected RNA-Seq libraries obtained from fibroblasts and whole blood. This design optimizes the reallocation of sequencing reads, enhancing coverage of rare, low-expressing transcripts and unique fusion events.The DepleteX High Expressing RNA Depletion kit is used during the RNA-seq library prep workflow starting with fibroblasts and whole blood samples after the ligation step and before the PCR step. This kit is compatible with most RNA-seq workflows; however, we recommend using it with the NEXTFLEX Rapid Directional RNA-seq kit 2.0.