BIOASSAY SYSTEMS
BioAssay Systems develops and markets innovative and high-throughput assay solutions to satisfy the ever increasing demands of the life sciences industry.
Bioassay Systems QuantiQuik™ Urea (BUN) Quick Test Strips. Direct determination of urea concentrations in food and beverage samples as well as biological samples.
For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.
Bioassay Systems QuantiChrom™ Hydroxyproline Assay Kit. For quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples.
For quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples. Key Features: Safe. Our hydroxyproline assay uses an improved perchlorate-free chemistry. Sensitive and accurate. Uses 20 µL sample. Linear detection range 0.5 µg/mL to 50 µg/mL hydroxyproline in 96-well plate assay. Convenient. Collagen can be hydrolyzed in Eppendorf tubes, and final incubation can be performed at 37°C. Method: OD560nm. Samples: Hydroxyproline and collagen in biologic and cosmetic samples. Species: All. Procedure: Assay takes Approx. 2 hrs. Kit size: 100 Tests. Detection limit: 0.5 µg/mL.
Bioassay Systems QuantiChrom™ HRP Detection Reagent. A stabilized sensitive reagent for peroxidases, for use in ELISA, immunochromogenic chemistry and Western blot.
A stabilized sensitive reagent for peroxidases, for use in ELISA, immunochromogenic chemistry and Western blot. Key Features: Highly sensitive. Detects as low as 7.6x10-8 U/well, or 7.7 pg/well horseradish peroxidase (HRP) in 96-well plate assays. Detects as low as 0.1 ppb Gliadin in ELISA. Improved stability and assay robustness. Method: OD450nm. Samples: peroxidases and HRP in ELISA. Species: All. Procedure: Assay takes Approx. 30 min. Kit size: 200 Tests. Detection limit: 76 nU/well, or 7.7 pg/well.
HORSERADISH PEROXIDASE (HRP) is an enzyme commonly used when conjugated to analytes of interest. When combined with hydrogen peroxide and a dye, HRP can be used to colorimetrically quantify the analyte.
Bioassay Systems QuantiChrom™ Total Protein Assay Kit. For quantitative determination of total protein.
For quantitative determination of total protein. Key Features: Fast and sensitive. Assay takes 10 min. Linear detection range of 5 - 200 mg/dL protein. Robust and Reliable. Substances in urine have minimal interference (<2%). Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD600nm (Pyrogallol Red). Samples: Urine, cerebrospinal fluid etc. Species: All. Procedure: Assay takes 10 min. Kit size: 1000 tests. Detection limit: 5 mg/dL.
Bioassay Systems CellQuanti-MTT™ Cell Viability Assay Kits. Homogeneous assay for cell viability, proliferation, cytotoxcity and evaluation of anticancer agents.
Homogeneous assay for cell viability, proliferation, cytotoxcity and evaluation of anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 950 cells can be accurately quantified. Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS. Z factors of 0.5 and above are observed. Can be readily automated with HTS liquid handling systems. Method: OD570nm. Samples: Cell culture. Species: All. Procedure: Assay takes 5 hrs, hands-on time 30 min. Kit size: 500 tests. Detection limit: 950 cells.
Bioassay Systems QuantiChrom™ Phytase Assay Kit. A simplest, direct "Add-and-Measure" assay for phytase enzyme activity in agricultural and biological samples.
A simplest, direct "Add-and-Measure" assay for phytase enzyme activity in agricultural and biological samples. Key Features: Simple and convenient: No complex detection reagents to mix. High sensitivity and wide detection range: Detection range of 0.01 to 20 U/L phytase in a 96-well plate assay. Fast and high-throughput: Homogeneous "mix-and-measure" assay allows for quantification of phytase activity within 60 minutes. Method: OD620nm. Samples: Enzyme extracts, agriculture and biological samples. Species: All (e.g. Wheat, Barley). Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 0.01 U/L.
Bioassay Systems Parallel Artificial Membrane Permeability Assay-BBB Kit. Assess blood brain barrier (BBB) membrane permeability of test compounds.
For quantitative determination of blood brain barrier (BBB) membrane permeability of test compounds. Key Features: Convenient. Includes all necessary equipment to run a PAMPA plate. Simple and low-cost. Procedure is easy to follow and more affordable than cell-based permeability assays. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: PAMPA. Samples: Chemical compounds. Procedure: Assay takes 20 hr, hands-on time 1 hr. Kit size: 96 tests.
Bioassay Systems EnzyChrom™ Glucose Assay Kit III. For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.
For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate: 0.03 to 2 mM (0.54 mg/dL to 36 mg/dL) glucose. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, saliva, milk, culture medium, food, beverages etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.03 mM.
Bioassay Systems QuantiQuik™ L-Glutamate Quick Test Strips. For rapid, direct determination of L-Glutamate (monosodium glutamate or MSG) concentrations in food and beverage samples as well as biological samples.
For rapid, direct determination of L-Glutamate (monosodium glutamate or MSG) concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 or 100 µL sample. Semi-quantitative measurement between 0-300 mg/L (undiluted) L-Glutamate. Convenient. No extra equipment required. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Soups, sauces, milk, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.
Bioassay Systems QuantiChrom™ LDH Cytotoxicity Assay Kit. For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium.
For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical trea™ent on cell lysis. Key Features: Safe. Non-radioactive assay (cf. chromium release assay). Fast. The whole procedure take 20 min. Robust and amenable to HTS. Single reagent, "mix-incubate-measure" type assay. High-throughput assay in 96-well plates allows simultaneous processing tens of thousands of samples per day. Method: OD500nm. Samples: Cell culture. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests.
Bioassay Systems EnzyLight™ Cytotoxicity Assay Kit. For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxicity and high-throughput screen for anticancer agents.
For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 50 cells can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells. Species: All. Procedure: Assay takes 2 min. Kit size: 100 tests. Detection limit: 50 cells.
Bioassay Systems QuantiChrom™ Biotin Assay Kit. Quantitative determination of biotin in food, cosmetic, and biotinylated proteins.
For quantitative determination of biotin in food, cosmetic, and biotinylated proteins. Key Features: Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well). Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD500nm. Samples: Food, cosmetics, supplements, and biotinylated proteins or antibodies. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 8 µM.
Bioassay Systems EnzyFluo™ Tryptophan Assay Kit. Direct determination of tryptophan in serum.
For direct determination of tryptophan in serum. Key Features: Fast and sensitive. Use of 10 µL sample. Linear detection range from 10 µM to 400 µM tryptophan in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 30 minutes. Room temperature assay. No 37 °C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm; Samples: Serum; Size: 100 wells.
Bioassay Systems QuantiChrom™ Arginase Inhibitor Screening Kit. HTS for inhibitor screening and evaluation of arginase inhibitors.
For evaluation and high-throughput screen (HTS) of arginase modulators. Key Features: Safe. Non-radioactive assay. High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in less than 2 hours and no 37°C heater is needed. Method: OD430nm. Samples: Arginase. Species: All. Procedure: Assay takes 1 hour 45 min. Kit size: 100 tests.
Bioassay Systems QuantiFluo™ Acid Phosphatase Assay Kit. Acid Phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)
For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.008 to 10 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.008 U/L.