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To clone a DNA fragment in pUC-linker and select colonies that have DNA inserts based on color selection.
Students will get to learn the effect of mutations in p53 tumor suppressor gene and its role in familial cancers.
To study gene structure, function, and to enhance gene expression.
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.
Prepare DNA molecular weight standards with Thermo Scientific™ phiX174 RF1 DNA, a 5386 bp double-stranded closed circular plasmid.
GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
Shipping Condition | Ambient, Wet Ice, Dry Ice |
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Content And Storage | Includes master mix, positive control, and water and accommodates the use of your own competent cells. |
Workflow Step | DNA Assembly |
Number of Fragments | Up to 6 fragments |
Product Type | HiFi Master Mix |
Control Type | Positive Control |
Cloning Method | Seamless cloning |
For Use With (Application) | Seamless Cloning & Genetic Assembly |
Product Line | GENEART™ |
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Product Type | Rapid DNA Ligation Kit |
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Enzyme | DNA Ligase |
The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
CAS: 9007-49-2 Molecular Formula: C15H31N3O13P2 Molecular Weight (g/mol): 0.00 MDL Number: MFCD00130921 InChI Key: AWBASQCACWFTGD-UHFFFAOYNA-N IUPAC Name: Deoxyribonucleic acid SMILES: *
CAS | 9007-49-2 |
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Molecular Weight (g/mol) | 0.00 |
MDL Number | MFCD00130921 |
SMILES | * |
IUPAC Name | Deoxyribonucleic acid |
InChI Key | AWBASQCACWFTGD-UHFFFAOYNA-N |
Molecular Formula | C15H31N3O13P2 |
The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15-60 minute isothermal reaction.
The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.