Miscellaneous Molecular Biology Reagents
Miscellaneous Molecular Biology Reagents
Invitrogen™ UltraPure™ Buffer-Saturated Phenol
UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.
Invitrogen™ Guanidine Isothiocyanate
UltraPure Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations.
Invitrogen™ UltraPure™ Dithiothreitol
UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups.
Invitrogen™ UltraPure™ Glycerol
UltraPure Glycerol is suitable for the concentration and storage of enzymes at low temperatures. A 50% (v/v) solution will not freeze at -20°C.
Invitrogen™ UltraPure™ BSA (50 mg/mL)
Ambion Ultrapure BSA is a 'non-acetylated' BSA pure enough to use when DNA or RNA integrity is critical.
Invitrogen™ Yeast tRNA (10 mg/mL)
Invitrogen Yeast tRNA purified from brewer's yeast. When used at a final concentration of 10 to 20 μg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids.
Invitrogen™ Sodium Acetate (3 M), pH 5.5, RNase-free
Invitrogen Molecular biology grade, 3M Sodium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ Ammonium Acetate (5 M), RNase-free
Invitrogen Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ SDS, 20% Solution, RNase-free
Invitrogen molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL.
Invitrogen™ IPTG (Dioxane-Free)
Invitrogen IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage.
Thermo Scientific™ Pierce™ D-Luciferin, Monopotassium Salt
Firefly luciferase substrate for in vitro and in vivo applications
Thermo Scientific™ Phenol:Chloroform + Tris Buffer
Perform DNA extractions with this phenol:chloroform:isoamyl alcohol mixture (25:24:1), accompanied by a buffer to equilibrate the solution at either pH 6.7 or pH 8.0.
Thermo Scientific™ X-Gal
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Thermo Scientific™ X-Gal Solution, ready-to-use
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Thermo Scientific™ DNA Loading Dye & SDS Solution (6X)
Perform agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins with this DNA loading dye containing SDS.