Classical and Basal Cell Culture Media

A variety of nutrient-rich solutions, including DMEM, RPMI, MEM, etc., used for maintaining and cultivating cells in laboratory settings, cell culture media plays a critical role in cell biology research, providing the necessary environment for cells to thrive and be studied in controlled laboratory conditions.

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Gibco™ RPMI 1640 Medium

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Form	Liquid
Without Additives	No HEPES,No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Leukemic Cells
With Additives	Glutamine,Phenol Red
Product Type	RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

Gibco™ RPMI 1640 Medium

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No HEPES,No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Leukemic Cells
With Additives	Glutamine,Phenol Red
Product Type	RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™, SynerGy™
Serum Level	Standard Serum Supplementation

Gibco™ DMEM/F-12, HEPES

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

With Additives	High Glucose,Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form	Liquid
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered

Gibco™ DMEM, high glucose, pyruvate, no glutamine

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, 293, Cos-7, and PC-12
Form	Liquid
Without Additives	No Glutamine,No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
With Additives	High Glucose,Phenol Red,Sodium Pyruvate
Product Type	DMEM (Dulbecco's Modified Eagle Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

Gibco™ MEM, NEAA, no glutamine

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
With Additives	Low Glucose,Phenol Red,Non Essential Amino Acids (NEAA)
Product Type	MEM (Minimum Essential Medium)
Without Additives	No Glutamine,No HEPES,No Sodium Pyruvate
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Product Line	Gibco™
Serum Level	Standard Serum Supplementation
Cell Type	Primary Rat Astrocytes

Gibco™ MEM (Richter's modification), no phenol red

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Pricing & Availability

Gibco™ Ham's F-12 Nutrient Mix, powder

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
With Additives	Glutamine,Phenol Red,Sodium Pyruvate
Product Type	Ham's F-12 Nutrient Mixture
Form	Powder
Without Additives	No HEPES,No Sodium Bicarbonate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco™
Cell Type	Primary Rat Astrocytes

Gibco™ MEM, HEPES, GlutaMAX™ Supplement

Minimum Essential Medium is commonly used of all cell culture media

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Sodium Pyruvate
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Rat Astrocytes
With Additives	Low Glucose,GlutaMAX,HEPES,Phenol Red
Product Type	MEM (Minimum Essential Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™, GlutaMAX™
Serum Level	Standard Serum Supplementation

Gibco™ MEM α, nucleosides, no phenol red

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No HEPES,No Phenol Red
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Rat Astrocytes
With Additives	Low Glucose,Glutamine,Sodium Pyruvate,Ribonucleosides,Deoxyribonucleosides
Product Type	MEM α (Minimum Essential Medium α)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™
Serum Level	Standard Serum Supplementation

Gibco™ Opti-MEM™ Reduced Serum Medium, powder

Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	Sp2, AE-1, CHO, BHK-21, and HEK
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 30 months from date of manufacture
With Additives	Glutamine,Phenol Red
Product Type	Opti-MEM I
Form	Powder
Classification	Animal Origin
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Shelf Life	30 Months From Date of Manufacture
Sterility	Sterile-filtered
Product Line	Gibco™, Opti-MEM™
Cell Type	Primary Fibroblasts

Gibco™ RPMI 1640 Medium, powder

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
Product Type	RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Form	Powder
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Shelf Life	36 Months From Date of Manufacture
Sterility	Sterile-filtered
Product Line	Gibco™
Serum Level	Standard Serum Supplementation
Cell Type	Leukemic Cells

Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	CHO, COS-7, and rat prostate epithelial cells
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Rat Astrocytes
With Additives	GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type	Ham's F-12 Nutrient Mixture
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco™, GlutaMAX™

Gibco™ Opti-MEM™ I Reduced Serum Medium

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	Sp2, AE-1, CHO, BHK-21, and HEK
Content And Storage	Store at 2–8°C and protect from light.
With Additives	Glutamine,Phenol Red
Product Type	Opti-MEM I
Form	Liquid
Classification	Human Origin
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Shelf Life	18 Months From Date of Manufacture
Concentration	1 X
Sterility	Sterile-filtered
Product Line	Gibco™, Opti-MEM™
Cell Type	Primary Fibroblasts

Gibco™ Glasgow's MEM, powder

Originally developed by Ian McPherson and Michael Stoker as modification of Eagle's medium

Pricing & Availability

Gibco™ Keratinocyte SFM (1X)

Contains L-glutamine.

Pricing & Availability

Classical and Basal Cell Culture Media

Classical and Basal Cell Culture Media

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Gibco™ RPMI 1640 Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM/F-12, HEPES Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM, high glucose, pyruvate, no glutamine Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM, NEAA, no glutamine Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM (Richter's modification), no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM, HEPES, GlutaMAX™ Supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM α, nucleosides, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Opti-MEM™ I Reduced Serum Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Keratinocyte SFM (1X) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ RPMI 1640 Medium

Gibco™ DMEM/F-12, HEPES

Gibco™ DMEM, high glucose, pyruvate, no glutamine

Gibco™ MEM, NEAA, no glutamine

Gibco™ MEM (Richter's modification), no phenol red

Gibco™ MEM, HEPES, GlutaMAX™ Supplement

Gibco™ MEM α, nucleosides, no phenol red

Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement

Gibco™ Opti-MEM™ I Reduced Serum Medium

Gibco™ Keratinocyte SFM (1X)