Complete kits designed to lyse cells and detect beta-galactosidase reporter protein activity in the prepared cell lysates, which can be obtained from mammals, yeasts, and other species. Also includes colorimetric and fluorometric detection assay reagents.
-Galactosidase or -D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains each constituting 1023 amino acids. It is very specific for D-galactose and requires K or Na and Mg2 to be fully active. -Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with -d-galactopyranosides.
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beta-Galactosidase EIA grade is a lyophilizate from E. coli overproducer consisting of enzyme protein phosphate buffer and sucrose. Substances which could interfere with the derivatization of NH2 or SH groups (e.g. 2-mercaptoethanol ammonium salts primary amines etc.) have been removed.
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This product is for research use only and not for diagnostic or therapeutic procedures. Customer is granted a limited, non-exclusive license for internal research and shall not attempt to reverse engineer. oNPG, beta-galactosidase substrate.
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-galactosidase (-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of -gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of -gal like O-nitrophenyl--D-galactopyranoside (ONPG). -gal is a cost-effective simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the -galactosidase gene LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
This product is for research use only and not for diagnostic or therapeutic procedures. Customer is granted a limited, non-exclusive license for internal research and shall not attempt to reverse engineer. oNPG, beta-galactosidase substrate.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
This product is for research use only and not for diagnostic or therapeutic procedures. Customer is granted a limited, non-exclusive license for internal research and shall not attempt to reverse engineer.
beta Galactosidase¿Staining¿Kit
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More