The NF-B/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-B. Acting as dimers, these transcription factors bind to B sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-B dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Caymans NF-B (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting human NF-B (p65) DNA binding activity in nuclear extracts and whole cell lysates. It will not cross-react with NF-B (p50).