Recombinant Protein;1MG;Human FGL1, Fc Tag (FG1-H5258) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Ile 312 (Accession # Q08830-1).;This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 60.5 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) at 10 μg/mL (100 μL/well) can bind Human FGL1, Fc Tag (Cat. No. FG1-H5258) with a linear range of 0.02-0.156 μg/mL (QC tested).;Serial dilutions of Anti-LAG3, Neutralizing Antibody were added into Human FGL1, Fc Tag (Cat. No. FG1-H5258): Biotinylated Human LAG-3, Mouse IgG2a Fc,Avitag (Cat. No. LA3-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.42303 μg/mL (Routinely tested).;FACS assay shows that Human FGL1, Fc Tag (Cat. No. FG1-H5258) can bind to 293T cells overexpressing human LAG3. The concentration of Human FGL1 is 3 μg/mL (Routinely test