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PCR Cloning Kits

PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.
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1 – 15 of 120 results
1

Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

Antibiotic Resistance Bacterial Ampicillin (AmpR)
Format Kit
Sample Type PCR Products
Cloning Method Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application) PCR Cloning
Vector pJET1.2/blunt
Product Line CloneJET
2
Promotions Available

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

3

Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells

Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)

Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Promoter T3, T7
Content And Storage Box 1: Store at -5 to -30°C.
• pCR XL-2-TOPO vector
• Salt Solution
• T3 Primer
• T7 Primer
• XL Control PCR Template
• XL Control PCR Primers
• Platinum SuperFi Green PCR Master Mix
• SuperFi GC Enhancer
• Nuclease-free water

Box 2: Store at room temperature.
• Gel Solubilization Buffer (L3)
• Binding Buffer (B2)
• Wash Buffer (W1)
• Elution Buffer (E1)
• PureLink Clean-up Spin Columns (in Wash Tubes)
• PureLink Recovery/Elution Tubes

Box 3:
• One Shot OmniMAX 2 T1R chemically competent E. coli
• S.O.C. Medium
• pUC 19 Control DNA

Store competent cells and pUC19 at -65 to -85°C.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method Zero Blunt TOPO
Bacterial or Yeast Strain OmniMAX2
For Use With (Application) PCR Cloning
Vector pCR-XL-2-TOPO
Product Line TOPO™
Cell Type Chemically Competent E. coli
5
Promotions Available

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing

Shipping Condition Dry Ice
Promoter T7, T3
Content And Storage Box 1:
• Topoisomerase I-activated pCR™4Blunt-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Vector TOPO Cloning Vectors
Product Line TOPO™
6
Promotions Available

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

7
Promotions Available

Invitrogen™ TOPO™ TA, Dual TOP10 (Supply Center Packaging)

For fast, efficient cloning and subsequent in vitro transcription/translation

8
Promotions Available

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Product Line TOPO™
9
Promotions Available

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent
10
Promotions Available

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Content And Storage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent
11
Promotions Available

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Packaging)

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

12
Promotions Available

Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products

13
Promotions Available

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells

For fast, efficient cloning and subsequent in vitro transcription/translation

Promoter T7, SP6
Content And Storage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent
14
Promotions Available

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

15
Promotions Available

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10 E. coli (Supply Center Packaging)

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning