ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry, which enables the highest yields and concentration of plasmid DNA (up to 3 g/l) directly from a spin-column. Coupling ZymoPURE with the innovative EndoZero™ II Spin-Columns, to eliminate endotoxins, achieves EndoZeroplasmid DNA ( 0.025 EU/g of plasmid DNA), making it suitable for transfection, restriction endonuclease digestion, in vivo studies, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.As an added convenience, the ZymoPURE™ II Plasmid Midiprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Syringe filters are included for rapid clearing of the lysate and the unique spin-column design allows the binding step to be performed using a vacuum or table top centrifuge.