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MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors.
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.
Shipping Condition | Dry Ice |
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Blue/White Screening | Yes (lacZΔM15) |
Cloning Unstable DNA | No |
Cloning Methylated DNA | Yes (mcrA) |
Transformation Efficiency Level | High Efficiency (>1 x 1010 cfu/μg) |
High-throughput Compatibility | Low |
Improves Plasmid Quality | Yes (endA1) |
Preparing Unmethylated DNA | No |
Plasmid | May be used for plasmids >20 kb |
Reduces Recombination | Yes (recA13) |
T1 Phage - Resistant (tonA) | Yes (Streptomycin) |
Antibiotic Resistance Bacterial | No |
Format | Tube |
Product Type | Electrocompetent Cells |
Species | E. coli (K12) |
Product Line | One Shot™ |
Contains F' Episome | No |
One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.
MultiShot FlexPlate DH10B T1R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.
MultiShot FlexPlate Stbl3 Chemically Competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity.
Convenient and accurate determination of the percentage of cells in each phase of the cell cycle
One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).
Align and calibrate Tali™ Image-Based Cytometer every three months
One Shot MAX Efficiency DH10B T1 Phage-Resistant E. coli have all the benefits of the DH10B genotype, with the addition of the tonA (also known as fhuA) genotype for resistance to T1 and T5 phage infection.
MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts.
Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that are used in many everyday cloning applications.
MAX Efficiency DH5αF'IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure of Hanahan. This strain is a derivative of the DH5α strain, sharing the same benefits.
ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/μg plasmid DNA.
One Shot BL21(DE3) Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.
The One Shot OmniMAX 2 T1R E. coli strain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 109 transformants/μg pUC19 DNA. It offers the highest transformation efficiency of any chemically competent cells in a One Shot format.