Crosslinking, Labeling and Protein Modification
Novus Biologicals™ 40nm Colloidal Gold Nanoparticles - 15OD
40nm colloidal gold nanoparticles ultra high quality, with narrow size distribution and uniform shape
Novus Biologicals™ InnovaCoat™ GOLD Antibody Labeling Kit, 80nm, Mini
Unique coated nanoparticle with a strongly bound protective surface coat
Molecular Probes™ Alexa Fluor™ 488 Azide (Alexa Fluor™ 488 5-Carboxamido-(6-Azidohexanyl), Bis(Triethylammonium Salt)), 5-isomer
The green-fluorescent Alexa Fluor™ 488 azide is reactive with terminal alkynes via a copper-catalyzed click reaction.
Fluorochrome Inc Fluoro-Gold, 50 mg
Hydroxystilbamidine (also called Fluoro-Gold) has been used extensively as a retrograde tracer for neurons and also a histochemical stain. Fluoro-Gold can undergo retrograde axonal transport, it shows extensive filling of dendrites, and has a high resistance to fading.
Bioworld Protein-A Gold™ (5-10 nm), 1 mL
Protein-A Gold Protein-A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus. It is capable of binding to the Fc portion of immunoglobulins, especially IgG from a large number of species. Protein-A may be conjugated with various reporter molecules, including colloidial gold, without affecting the antibody binding site on the molecule. Specifications:Particle size5-10 nm (monodisperse), nominal 15 nm (colloidal gold) Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, 20% glycerol, and 15 mM sodium azide. Storage: 2-8 C
Nanocomposix Ultra Uniform Gold Nanospheres - PEG-Carboxyl - 30 nm, 0.05 mg/mL in aq. 2 mM sodium citrate, 5 mL
As a global leader in gold nanoparticle production, nanoComposix specializes in providing high-purity and monodisperse nanomaterials -- delivering the quality you need for consistent results.30 nm Ultra Uniform spherical gold nanoparticles combine nearly perfect spherical shape, narrow size distribution, and strong plasmonic optical properties. These nanoparticles are useful in a broad range of applications from reference standards for size and particle number, biological labels, nanomedicine, drug delivery, biosensors, and more.nanoComposix provides high quality nanomaterials that are precisely engineered and highly characterized. All products are supplied with batch-specific CoA's including TEM, DLS, Zeta, & UV-Vis characterization data.Intended for Research Use Only. Contact us for custom formulations, ISO 13485/cGMP compliant materials, or bulk supply requirements.
Gold Biotechnology Inc TCEP-HCl 10 g
TCEP (Tris (2-Carboxyethyl) phosphine Hydrochloride) is an odorless reducing agent suitable for protein applications and in the preparation for electrophoresis. TCEP is water soluble, is stable in a variety of solutions and is particularly useful in the labeling of cysteine residues with maleimides, preventing the cysteines from forming disulfide bonds.
Gold Biotechnology Inc Guanidine thiocyanate (Guanidine Isothiocyanate) 1 kg
During cell lysis and the extraction of DNA or RNA, denaturing enzymes such as DNase and RNase are released. To prevent DNA or RNA denaturation, the chaotropic agent, Guanadine thiocyanate is the preferred agent for use during cell lysis, and for DNA or RNA extraction. This is because of its strong ability to irreversibly inhibit the activity of DNase and RNase.
Gold Biotechnology Inc Guanidine HCl 5 kg
Guanidine HCl is a freely soluble chaotropic agent routinely used for protein denaturation and folding.
Gold Biotechnology Inc Phenylmethylsulfonyl fluoride (PMSF) 25 g
PMSF is a serine protease inhibitor that irreversibly inhibits serine proteases by sulfonylation of the serine residue in the active site of the protease. It is widely used in protocols for the isolation of proteins.
Gold Biotechnology Inc Proteinase K, RNase/DNase free 1 g
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.
Gold Biotechnology Inc DTT (Dithiothreitol) (> 99% pure) Protease free 25 g
Dithiothreitol (DTT) is the common name of the more popular of the two Cleland’s Reagents (the other being Dithioerythritol or DTE). DTT is a powerful reducing agent that forms a stable six-membered ring with an internal disulfide bond which is resistant to oxidation. DTT is often used for the following: reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl) cystamine to break apart the matrix of a polyacrylamide gel, the reduction of disulfide bonds in proteins, the prevention of those bonds from forming between cysteine residues and lastly, DTT is often used to reduce thiolated DNA in order to minimize dimerization.
Creative Pegworks HS-PEG-SH, MW 20k - 5g
HS-PEG-SH is a linear homobifunctional PEG with two reactive free thiol. SH, sulfhydryl, or mercapto groups selectively react with maleimide and transition metal surface including gold, silver. HS-PEG-SH undergoes air oxidation and polymerization forming S-S disulfide bonds. Disulfide bond is reversible with reducing agents such as TECP. It is a useful reagent for reversible crosslinking PEGylation. HS-PEG-SH can crosslink two molecules containing maleimide. Thiol is very useful in thiol-ene reaction to react with acrylate, norbornene etc. Bifunctional thiol PEG is a common crosslinker in preparing hydrogels.
Creative Pegworks HS-PEG-SH, MW 10k - 5g
HS-PEG-SH is a linear homobifunctional PEG with two reactive free thiol. SH, sulfhydryl, or mercapto groups selectively react with maleimide and transition metal surface including gold, silver. HS-PEG-SH undergoes air oxidation and polymerization forming S-S disulfide bonds. Disulfide bond is reversible with reducing agents such as TECP. It is a useful reagent for reversible crosslinking PEGylation. HS-PEG-SH can crosslink two molecules containing maleimide. Thiol is very useful in thiol-ene reaction to react with acrylate, norbornene etc. Bifunctional thiol PEG is a common crosslinker in preparing hydrogels.
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