Search results for "Enzo Life Sciences"
Enzo Life Sciences alpha Gal Epitope (Galalpha1-3Galbeta1-4GlcNAc-R) monoclonal antibody (M86) (1 Vial)
Clone: M86. Host: Mouse. Isotype: IgM. Immunogen: Rabbit red blood cell membrane. Source: Hybridoma tissue culture. Species Reactivity: Mouse, Rat, Porcine. Specificity: Recognizes synthetic and naturally produced mouse, rat and pig Galα1-3Gal epitopes on glycoproteins and glycolipids. Crossreactivity: Does not cross-react with β-Gal glycoproteins or BSA. Applications: ELISA. Flow Cytometry, IHC (PS), WB. FUNC. Recommended dilutions: Note: Do not use non-fat milk for blocking! Many blocking agents from non-human mammal. Sources will cross-react with the antibody. Appropriate antibody dilution may vary from lot to lot. An initial dilution of 1:5 is recommended. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. Formulation: Liquid. Tissue culture supernatant. Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Enzo Life Sciences IM-12, (5mg), CAS Number: 1129669-05-1
GSK-3β inhibitor (IC50=53nM (enzymatic), 3.8µM (cellular)). The compound significantly increases β-catenin levels, leads to TCF-induction and promotes neuronal differentiation in human neural progenitor cells via activation of canonical Wnt signaling. Alternative Name: 3-(4-Fluorophenylethylamino)-1-methyl-4-(2-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione. Formula: C22H20FN3O2. MW: 377.4. CAS Number: 1129669-05-1. Purity: ≥98% (HPLC). Appearance: Orange solid. Solubility: Soluble in DMSO (25 mg/ml) or in 100% ethanol (5 mg/ml). Use/Stability: Store as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. Long Term Storage: -20°C
Enzo Life Sciences BIOMOL Green (1x1L)
A simple and convenient method for colorimetric phosphate quantitation. Applications: FUNC. Colorimetric detection, HTS. Contents: Biomol Green Reagent (Prod. No. BML-AK111) (1l or 250ml; liquid in screw cap plastic bottle) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects. Phosphate Standard (Prod. No. BML-KI102) (1ml; 800µm phosphate in distilled water) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects.
Enzo Life Sciences Streptozotocin, (1g), CAS Number: 18883-66-4
O-GlcNAcase inhibitor. Widely used diabetes inducer in rodents. Inhibition of β-cell O-GlcNAcase by streptozotocin is the mechanism that accounts for its diabetogenic toxicity. N-nitroso-containing antibiotic, acting as a nitric oxide (NO) donor and vasorelaxant. Relaxes phenylephrine-contracted aortic rings at 10µM. Potent methylating agent for DNA. Alternative Name: Streptozocin, 2-Deoxy-2-(3-methyl-3-nitrosoureido)-D-glucopyranose, STZ. Formula: C8H15N3O7. MW: 265.2. CAS Number: 18883-66-4. MI: 14: 8832. Purity: ≥97% (Assay). Appearance: White to pale yellow crystalline solid. Solubility: Soluble in water (nH2O at 102.8mg/ml) or 100% ethanol (200 proof at 0.92mg/ml). Use/Stability: Because solutions of the compound spontaneously give off NO gas at room temperature, we recommend that solutions be made immediately before use. Long Term Storage: -20°C
Enzo Life Sciences Proteasome 20S (human), (purified) (50 µg)
High integrity preparation for use in proteasome research. Formulation: Liquid. In 20mM TRIS-HCl, pH 7.2, containing 1mM EDTA, 1mM DTT, 1mM sodium azide and 50% glycerol. Source: Isolated and purified from human erythrocytes. Use/Stability: 20S Proteasome remains relatively stable and active so long as repetitive freeze-thaw cycles are avoided. It has been demonstrated that 20S proteasome undergoes only slow loss of activity when stored at 4°C for up to six months. If it is to be used on a regular basis, it is recommended that the material should be stored between 0°C-4°C during the duration of use. For longer term storage the material may be dispensed as single use aliquots and stored at -80°C. Handling: Avoid freeze/thaw cycles. Long Term Storage: -80°C
Enzo Life Sciences Auranofin, (100mg), CAS Number: 34031-32-8
Anti-arthritic agent. Alternative Name: SKF 39162, 1-Thio-beta-D-glucopyranosatotriethyl phosphine gold-2,3,4,6-tetraacetate. Formula: C20H34AuO9PS. MW: 678.5. CAS Number: 34031-32-8. MI: 14: 878. Purity: ≥98% (HPLC). Appearance: White solid. Solubility: Soluble in DMSO (5mg/ml) or 100% ethanol (4mg/ml). Use/Stability: Stable for at least 1 year after receipt when stored, as supplied, at room temperature. Stock solutions are stable for up to 3 months at -20°C. Long Term Storage: Ambient
Enzo Life Sciences Collagen I, rat tail (100 mg)
Alternative name: COL1. MW: 235kDa, 215kDa, 130kDa, 115kDa. Concentration: 5mg/ml. Endotoxin content: <100 EU/mg purified protein (LAL test). Purity: ≥90% (SDS-PAGE). Formulation: Sterile liquid. In 0.02N acetic acid. Appearance: Hazy viscous liquid. Source: Isolated from rat tail tendons. Handling: Keep sterile. Do not freeze. Long Term Storage: +4°C
Enzo Life Sciences Cellular senescence activity assay (1x120assays)
Highly reproducible SA-β-gal activity kit. The 96-well Cellular senescence assay kit provides an easy-to-use and efficient method to determine the cellular senescence by measuring SA-β-gal activity using a fluorometric substrate. This quantitative assay uses cell lysate for both SA-β-galactosidase activity determination and normalization of samples containing different cell numbers. Each kit provides sufficient quantities to perform up to 120 assays in a 96-well plate. Applications: Fluorescent detection, HTS. Contents: 2X Cell lysis buffer. 2X Reaction buffer. SA-β-gal substrate (20X). Stop solution
Enzo Life Sciences Caspase-1 (human), (recombinant) (5000 U)
Highly active caspase essential for apoptosis. Alternative name: Interleukin 1 beta converting enzyme, IL-1 beta converting enzyme, ICE. MW: ~20 + 10kDa. Concentration: 100 U/µl. Purity: ≥90% (SDS-PAGE). Formulation: Liquid. In 50mM HEPES, pH 7.4, containing 100mM sodium chloride, 0.5% CHAPS, 1mM EDTA, 10% glycerol and 10mM DTT. Source: Produced in E. coli. cDNA encodes residues identical to Asn120-His404 (C-terminus) at Genbank Accession No. M87507, except for an Asp381 to Glu change, introduced to stabilize the enzyme against autoproteolysis.
Enzo Life Sciences SAG, (1mg), CAS Number: 364590-63-6
Smoothened ligand. A potent cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM). Shown to induce Hedgehog pathway activation (EC50= ~3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine (BML-GR334) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations. Formula: C28H28ClN3OS. MW: 490.1. CAS Number: 364590-63-6. Purity: ≥95% (HR-MS, 1H-NMR, 13C-NMR). Appearance: White to off-white solid. Solubility: Soluble in DMSO (10mg/ml), sparingly soluble in water (acidification with HCl increases water solubility). Use/Stability: Stable for at least 6 months when stored at -20°C. Long Term Storage: -20°C
Enzo Life Sciences BIOMOL Green (1x250ml)
A simple and convenient method for colorimetric phosphate quantitation. Applications: FUNC. Colorimetric detection, HTS. Contents: Biomol Green Reagent (Prod. No. BML-AK111) (1l or 250ml; liquid in screw cap plastic bottle) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects. Phosphate Standard (Prod. No. BML-KI102) (1ml; 800µm phosphate in distilled water) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects.
Enzo Life Sciences Digoxigenin NHS ester, (1mg), CAS Number: 129273-26-3
Digoxigenin is a non-radioactive immune-tag, widely used for in situ hybridization and protein labeling. Pairing with anti-digoxigenin antibodies, digoxigenin tag allows the detection of interested biomolecules with high affinity and specificity. Digoxigenin NHS ester reacts with amino groups within biomolecules (nucleic acid, proteins, etc) and enables the attachment of digoxigenin into these molecules. Formula: C35H50N2O10. MW: 658.78. CAS Number: 129273-26-3. Purity: ≥80% (HPLC). Appearance: Off-white solid. Solubility: Soluble in DMSO or DMF. Use/Stability: Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. Long Term Storage: -20°C
Enzo Life Sciences Spectinomycin . sulfate, (10g), CAS Number: 64058-48-6
Aminocyclitol antibiotic. Antibiotic. Inhibits protein synthesis (elongation) by interfering with peptidyl tRNA translocation. Effective against Gram-negative and Gram-positive bacteria (Gonnococcus only). Mutation in rpsE (the gene for ribosomal protein S5) prevents binding of spectinomycin. Alternative Name: U-18409E. Formula: C14H24N2O7. H2SO4. MW: 430.4. CAS Number: 64058-48-6. Purity: ≥98% (HPLC). Appearance: White solid. Solubility: Soluble in water. Long Term Storage: -20°C
Enzo Life Sciences Sphingosine-1-phosphate, D-erythro, (1mg), CAS Number: 26993-30-6
Endogenous signaling molecule. Formula: C18H38NO5P. MW: 379.5. CAS Number: 26993-30-6. Purity: ≥98% (TLC). Solubility: Sparingly soluble in 1:1 ethanol:water. Addition of small amounts of acetic acid may help dissolve this product. Soluble in warm methanol. For delivery of sphingosine-1-phosphate to cells, prepare a 125μM stock in 4mg/ml BSA as follows: Dissolve sphingosine-1-phosphate (S-1-P) in methanol (0.5 mg/ml). This may require boiling (65°C), with occasional replacement of evaporated methanol. Crushing of solid prior to addition of methanol and crushing and stirring during heating is helpful. Aliquot desired amounts of methanol stock to tubes. Evaporate the solvent with a stream of nitrogen, swirling to deposit a thin film on the inside of the tube. Aliquots may be stored at -20 °C at this point or dissolved with enough 4mg/ml BSA (fatty acid free Bovine Serum Albumin in water; 37°C, 30 min. with repeated vortexing) to make 125 µM stock. Long Term Storage: -20°C
Enzo Life Sciences MULTIVIEW (mouse-HRP/rabbit-AP) IHC kit (1x100tests)
Superior multiplex IHC kit for efficient detection of multiple antigens in a single tissue sample. The MULTIVIEW (mouse-HRP/rabbit-AP) IHC kit is a non-biotin one-step detection system suitable for demonstrating antigens in formalin-fixed paraffin-embedded tissues and cryostat sections. The kit may also be used with blood smears, cytosmears, and cell preparations. Assay Time: ~2 hours. Applications: IHC. Contents: Peroxidase Block, POLYVIEW IHC reagent (mouse-HRP), POLYVIEW IHC reagent (rabbit-AP), HIGHDEF IHC chromogen substrate (DAB, stable) buffer & chromogen, HIGHDEF red IHC chromogen (AP) buffer & chromogen, Empty mixing bottles